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Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
Published on: June 16, 2021
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TBPL2/TFIIA complex establishes the maternal transcriptome through oocyte-specific promoter usage.
Changwei Yu1,2,3,4, Nevena Cvetesic5, Vincent Hisler1,2,3,4
1Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404, Illkirch, France.
Nature Communications
|December 23, 2020
Summary
During oocyte development, TATA-binding protein-like 2 (TBPL2) replaces TBP to mediate essential transcription. TBPL2 utilizes a specific recognition code for transcription start sites, establishing the oocyte transcriptome.
Area of Science:
- Molecular Biology
- Developmental Biology
- Genetics
Background:
- Oocyte growth requires transcription for maternal RNA and protein reserves, crucial for developmental competence.
- General transcription factors, like TATA-binding protein (TBP), are vital for RNA polymerase II transcription.
Purpose of the Study:
- To investigate the role of TBP paralogue TBPL2 in oocyte transcription.
- To determine how TBPL2 mediates transcription and establishes the oocyte transcriptome.
Main Methods:
- Transcriptome analysis to identify TBPL2-mediated genes.
- Transcription start site (TSS) mapping to analyze promoter utilization.
- Analysis of TBPL2 complex formation.
Main Results:
- TBPL2 does not form a canonical TFIID complex in oocytes.
- TBPL2 drives transcription of oocyte-specific genes and endogenous retroviral elements.
- TBPL2/TFIIA complex exhibits a preference for TATA-like motifs, ensuring sharp TSS selection.
Conclusions:
- TBPL2 plays a critical role in establishing the oocyte transcriptome.
- TBPL2 employs a distinct TSS recognition code compared to TBP/TFIID.
- The TBPL2/TFIIA complex is essential for precise transcription initiation during oogenesis.
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