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Nucleotide sequence of the type A staphylococcal enterotoxin gene
1Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Journal of Bacteriology
|January 1, 1988
Summary
The staphylococcal enterotoxin A (entA) gene sequence was determined, revealing its precursor and mature forms. DNA hybridization showed significant sequence divergence within the staphylococcal enterotoxin family.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Staphylococcal enterotoxins (SEs) are a group of related toxins produced by Staphylococcus aureus.
- Understanding the genetic basis of SEs is crucial for identifying and controlling their production.
Purpose of the Study:
- To determine the nucleotide sequence of the staphylococcal enterotoxin A (entA) gene.
- To analyze the homology between entA and genes encoding other SEs.
- To investigate sequence divergence within the SE family.
Main Methods:
- Nucleotide sequencing of the entA gene.
- Computer analysis of amino acid sequence homology.
- DNA-DNA hybridization using entA-derived probes.
Main Results:
- The entA gene is 771 base pairs, encoding a 257-amino acid precursor.
- Mature staphylococcal enterotoxin A shares significant homology with other SEs and related toxins.
- DNA hybridization revealed considerable sequence divergence among different SE genes.
Conclusions:
- The entA gene sequence provides insights into the structure and evolution of staphylococcal enterotoxins.
- Sequence divergence within the SE family suggests distinct evolutionary pathways.
- This study contributes to the understanding of SE diversity and potential for cross-reactivity.