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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Nov 24, 2025

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

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Microarray based gene expression profiling of advanced gall bladder cancer.

A Kumar¹, R Gupta¹, N Mathur¹

¹Dr. B.R.A., Institute-Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi 110029, India.

Experimental Oncology

|December 28, 2020

Summary

This summary is machine-generated.

Gall bladder cancer (GBC) involves complex molecular mechanisms. This study identified differentially expressed genes, including Cdc45 and MCM4, suggesting their oncogenic role and potential as therapeutic targets in advanced GBC.

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Area of Science:

Oncology
Molecular Biology
Genomics

Background:

Gall bladder cancer (GBC) is aggressive, with unknown molecular drivers.
GBC shows predilection for females and specific geographic regions.

Purpose of the Study:

To comprehensively analyze differentially expressed genes in advanced GBC versus chronic cholecystitis (CC).
To identify potential molecular targets for GBC diagnosis and therapy.

Main Methods:

Microarray analysis of 12 advanced GBC and 4 CC fresh tissue specimens.
In silico analysis and immunohistochemistry validation of selected genes (TPX2, Cdc45, MCM4).

Main Results:

Identified 1307 differentially expressed genes (535 upregulated, 772 downregulated) in advanced GBC vs CC.
Key affected pathways include cell cycle, DNA replication, and oxidative stress.
Cdc45 and MCM4 proteins showed significant positive association in advanced GBC (p=0.043).

Conclusions:

The Cdc45-MCM4 axis is potentially regulated in advanced GBC.
Differentially expressed genes like TPX2, Cdc45, and MCM4 may serve as future diagnostic or therapeutic targets.