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Human serum alpha 2HS-glycoprotein modulates in vitro bone resorption.

G C Colclasure1, W S Lloyd, M Lamkin

  • 1Department of Biochemistry, Boston University School of Medicine, Massachusettes 02118.

The Journal of Clinical Endocrinology and Metabolism
|January 1, 1988
PubMed
Summary

Normal human serum alpha-2HS glycoprotein (alpha 2HS) exhibits a biphasic effect on bone resorption. It stimulates calcium release at low doses but inhibits it at higher concentrations, suggesting a role in bone metabolism regulation.

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Area of Science:

  • Biochemistry
  • Bone Biology
  • Endocrinology

Background:

  • Bone resorption is a critical process in skeletal remodeling and is implicated in various pathologies.
  • Previous isolation of bone-resorbing proteins from cancer ascites fluid revealed a relationship to alpha-2HS glycoprotein (alpha 2HS).

Purpose of the Study:

  • To investigate the effects of normal human serum alpha-2HS glycoprotein on bone resorption in vitro.
  • To determine the dose-dependent activity of alpha 2HS on calcium release from bone.

Main Methods:

  • Purification of normal human serum alpha-2HS glycoprotein.
  • In vitro assessment of 45Ca2+ release from bone across a range of alpha 2HS concentrations.

Main Results:

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  • Normal human serum alpha 2HS demonstrated a biphasic effect on bone resorption.
  • Low concentrations (up to 40 µg/mL) stimulated 45Ca2+ release, with a maximum effect observed at 40 µg/mL (ratio 2.5 ± 0.30).
  • Higher concentrations (above 40 µg/mL) led to a dose-dependent inhibition of calcium mobilization, returning to baseline above 80 µg/mL.

    • Conclusions:

    • Serum alpha 2HS glycoprotein plays a significant role in regulating bone metabolism.
    • The observed biphasic response suggests a complex regulatory mechanism for bone resorption involving alpha 2HS.