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Spatiotemporal Patterning of Living Cells with Extracellular DNA Programs.

Marc Van Der Hofstadt1, Jean-Christophe Galas1, André Estevez-Torres1

  • 1Sorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Laboratoire Jean Perrin (LJP), F-75005, Paris, France.

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|December 28, 2020
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Summary
This summary is machine-generated.

This study introduces enzyme-DNA molecular programs in extracellular media for spatiotemporal control of cell internalization. These programs enable precise timing and positioning of cellular responses, mimicking developmental processes.

Keywords:
DNA nanotechnologycell patterninggrowth mediumreaction−diffusionreactive extracellular media

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Area of Science:

  • Synthetic Biology
  • Biochemistry
  • Cell Biology

Background:

  • Current extracellular reaction networks lack feedback and out-of-equilibrium dynamics for spatiotemporal control.
  • Engineering extracellular environments to precisely control intracellular composition is challenging.

Purpose of the Study:

  • To develop functional enzyme-DNA molecular programs in extracellular media for spatiotemporal control of cellular processes.
  • To demonstrate the ability to encode temporal and positional information into living cells using synthetic extracellular programs.

Main Methods:

  • Utilized enzyme-DNA molecular programs within an extracellular medium supporting human cell growth.
  • Constructed an internalization program to deliver fluorescent DNA into cells, maintaining functionality for at least 48 hours.
  • Engineered a spatially inhomogeneous program to generate tunable reaction-diffusion patterns for cell internalization.

Main Results:

  • Demonstrated functional enzyme-DNA programs in extracellular media for controlling cell internalization.
  • Achieved precise temporal and positional control over cell internalization using nonequilibrium dynamics.
  • Generated a tunable two-band pattern of cell internalization via reaction-diffusion dynamics.

Conclusions:

  • Synthetic extracellular programs can provide temporal and positional information to living cells, emulating developmental mechanisms.
  • Nonequilibrium reactive extracellular media offer potential applications in biomolecular tracking, tissue engineering, and smart bandages.