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TNF-α detection using gold nanoparticles as a surface-enhanced Raman spectroscopy substrate.

Elizabeth Loredo-García1, Alejandra Ortiz-Dosal2, Juan Manuel Núñez-Leyva2

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|December 28, 2020
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Summary

This study demonstrates Surface-Enhanced Raman Spectroscopy (SERS) for detecting Tumor Necrosis Factor-alpha (TNF-α). Gold nanoparticles enabled sensitive detection of TNF-α at very low concentrations.

Keywords:
SERSTNF-αbiomarkerscytokinesgold nanoparticles

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Area of Science:

  • Biochemistry
  • Spectroscopy
  • Nanotechnology

Background:

  • Tumor Necrosis Factor-alpha (TNF-α) is a key cytokine implicated in inflammatory processes.
  • Surface-Enhanced Raman Spectroscopy (SERS) offers potential for sensitive biomolecule detection.
  • Gold nanoparticles (AuNPs) are explored as effective SERS substrates.

Purpose of the Study:

  • To identify TNF-α in aqueous solutions using AuNPs as a SERS substrate.
  • To establish a SERS-based method for quantifying TNF-α.

Main Methods:

  • Raman and SERS spectra were acquired for varying TNF-α concentrations.
  • AuNPs were synthesized and characterized using dynamic light scattering and transmission electron microscopy.
  • Optical transmission spectroscopy was employed for sample analysis.

Main Results:

  • Characterization confirmed AuNP size around 9.6 nm.
  • Observed Raman bands correlated with aromatic amino acid side chains in TNF-α.
  • SERS detected TNF-α at concentrations as low as 0.125 pg/ml.

Conclusions:

  • SERS successfully detected TNF-α in aqueous solutions.
  • The method achieved high sensitivity, detecting TNF-α at physiologically relevant concentrations.
  • AuNPs serve as a viable substrate for SERS-based TNF-α detection.