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Up-regulated miR-155 is associated with poor prognosis in childhood acute lymphoblastic leukemia and promotes cell

Cong Liang1, Yu Li1, Li-Na Wang1

  • 1Department of Pediatrics, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.

Hematology (Amsterdam, Netherlands)
|December 28, 2020
PubMed

Insights

High miR-155 expression predicts poor prognosis in childhood acute lymphoblastic leukemia (ALL). This microRNA promotes ALL cell proliferation by targeting ZNF238, suggesting miR-155 as a potential prognostic biomarker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Acute lymphoblastic leukemia (ALL) is a common childhood cancer.
  • Identifying prognostic biomarkers is crucial for improving patient outcomes.

Purpose of the Study:

  • To identify a novel microRNA (miRNA) for predicting the prognosis of childhood ALL.
  • To explore the underlying mechanism of the identified miRNA in ALL.

Main Methods:

  • Analysis of miRNA expression profiles from public databases (GEO).
  • RT-PCR to measure miR-155 expression in ALL patients.
  • In vitro studies using ALL cell lines to assess the effects of miR-155.
  • Dual-luciferase reporter assay to confirm miR-155 target.
  • MTT and flow cytometry to evaluate cell proliferation and apoptosis.

Main Results:

  • miR-155 was significantly upregulated, while ZNF238 was downregulated in diagnosed ALL.
  • High miR-155 expression correlated with poor prognosis.
  • Overexpression of miR-155 enhanced ALL cell proliferation and inhibited apoptosis.
  • miR-155 directly targets and downregulates ZNF238.
  • Silencing ZNF238 promoted proliferation in ALL cells.

Conclusions:

  • miR-155 shows potential as a biomarker for predicting childhood ALL prognosis.
  • miR-155 promotes ALL cell proliferation by targeting ZNF238.
  • ZNF238 may function as a tumor suppressor in childhood ALL.
Abstract

Keywords:
MiR-155ZNF238acute lymphoblastic leukemiaprognosisproliferation‌children‌miRNA‌treatment

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