Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A structurally reassembled sunflower stem pith composite sponge for prehospital temporary emergency hemostasis.

Biomaterials science·2026
Same author

A Novel Simplified Prognostic Model for 90-day Transplant-free Survival in Patients with Severe Acute Liver Injury/Acute Liver Failure.

Journal of clinical and translational hepatology·2026
Same author

IRF7 links HK1-dependent histone lactylation to fibroblast activation and cardiac fibrosis.

EMBO molecular medicine·2026
Same author

PA-X I94V mutation modulates the pathogenicity of the highly pathogenic H7N9 influenza A virus in mice and chickens.

Veterinary microbiology·2026
Same author

Organ failure-based clinical classification at onset of acute-on-chronic liver failure: a prospective multicenter cohort study.

Scientific reports·2026
Same author

Zinc-chelated cuttlefish ink melanin nanoparticle-complexed sprayable chitosan-based hydrogel for promoting infected wound healing.

Biomaterials science·2026

Related Experiment Video

Updated: Nov 24, 2025

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
12:00

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

Published on: January 22, 2015

12.7K

Temperature sensitive self-assembling hydroxybutyl chitosan nanoparticles with cationic enhancement effect for

Shichao Bi1, Ming Kong2, Xiaojie Cheng2

  • 1College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao, 266003, Shandong Province, China; Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266000, China.

Carbohydrate Polymers
|December 28, 2020
PubMed
Summary

Hydroxybutyl chitosan (HBC) nanoparticles exhibit temperature-dependent assembly and poly-cationic structures. These versatile nanoparticles show excellent contaminant removal and promote cell proliferation, indicating broad multi-functional applications.

Keywords:
Cationic enhancement effectHydroxybutyl chitosanMulti-functional applicationsNanoparticlesSelf-assemblingTemperature sensitive

More Related Videos

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications
05:26

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications

Published on: April 13, 2022

3.7K
Solubility of Hydrophobic Compounds in Aqueous Solution Using Combinations of Self-assembling Peptide and Amino Acid
05:08

Solubility of Hydrophobic Compounds in Aqueous Solution Using Combinations of Self-assembling Peptide and Amino Acid

Published on: September 20, 2017

17.3K

Related Experiment Videos

Last Updated: Nov 24, 2025

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
12:00

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

Published on: January 22, 2015

12.7K
Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications
05:26

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications

Published on: April 13, 2022

3.7K
Solubility of Hydrophobic Compounds in Aqueous Solution Using Combinations of Self-assembling Peptide and Amino Acid
05:08

Solubility of Hydrophobic Compounds in Aqueous Solution Using Combinations of Self-assembling Peptide and Amino Acid

Published on: September 20, 2017

17.3K

Area of Science:

  • Materials Science
  • Polymer Chemistry
  • Biotechnology

Background:

  • Chitosan derivatives offer tunable properties for advanced applications.
  • Developing novel materials with controlled self-assembly is crucial.
  • Hydroxybutyl chitosan (HBC) presents unique characteristics for material design.

Purpose of the Study:

  • To synthesize and characterize hydroxybutyl chitosan (HBC) with varying degrees of substitution (DS).
  • To investigate the temperature-dependent reversible morphological transition and self-assembly mechanism of HBC nanoparticles.
  • To evaluate the functional properties of HBC nanoparticles, including contaminant removal and cytocompatibility.

Main Methods:

  • Homogeneous reaction system (KOH/urea) for HBC synthesis.
  • Spectroscopic and thermal analysis (FTIR, NMR, XRD, TGA) for structural characterization.
  • Microscopy and dynamic light scattering (TEM, AFM, DLS) for morphology and assembly studies.
  • Functional assays for coagulation, bacteriostasis, superoxide anion clearance, anionic contaminant removal, and cytocompatibility.

Main Results:

  • HBCs with different DS were successfully synthesized.
  • HBC nanoparticles demonstrated temperature-dependent reversible morphological transition and formed poly-cationic structures upon assembly.
  • HBC nanoparticles exhibited excellent flocculation and removal of anionic composites, alongside significant bacteriostatic and superoxide anion clearance capabilities.
  • Cytocompatibility tests revealed that HBC effectively promoted the proliferation of fibroblasts and mesenchymal stem cells.

Conclusions:

  • The synthesized HBCs possess tunable properties and self-assembly capabilities.
  • HBC nanoparticles show significant potential for environmental remediation due to their contaminant removal efficacy.
  • The cytocompatibility of HBC nanoparticles suggests their utility in biomedical applications, particularly in promoting cell growth and division.