NBIGV-DB: A dedicated database of non-B cell derived immunoglobulin variable region

Area of Science:

• Immunology
• Bioinformatics
• Genomics

Background:

• Immunoglobulins (Igs) are crucial for antigen recognition, traditionally attributed to B lymphocytes (B-Igs).
• Emerging evidence indicates non-B cells, including epithelial, neuronal, germ, and myeloid cells, also produce Igs (non-B Igs).
• Non-B Igs possess distinct cellular functions beyond antibody activity and exhibit conserved V(D)J recombination patterns, contrasting with the high diversity of B-Igs.

Purpose of the Study:

• To establish a comprehensive database (NBIGV) for analyzing and comparing immunoglobulin variable region sequences from both B and non-B cells.
• To provide a centralized resource for researchers studying the diversity and function of non-B Igs.

Main Methods:

• Compilation of a non-B Ig variable region repertoire from mouse samples, including 727,989 V_HDJ_H and VκJκ recombination sequences.
• Development of a web-accessible database (NBIGV) enabling users to search, browse, and download non-B Ig sequences.
• Integration of NCBI-BLAST for sequence comparison against known non-B Ig sequences.

Main Results:

• The NBIGV database contains a substantial repertoire of non-B Ig variable region sequences from various mouse strains and tissues.
• Users can query the database by mouse strain, tissue, and Ig class.
• The database facilitates sequence analysis, comparison, and download, supported by supplementary sample information and a submission portal.

Conclusions:

• The NBIGV database serves as a valuable, freely accessible resource for the analysis of non-B Ig sequences.
• It supports research into the distinct characteristics and functions of immunoglobulins produced by non-B cell lineages.
• Facilitates comparative studies between B-cell and non-B-cell derived immunoglobulins.