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N-terminomics - its past and recent advancements
Prashant Kaushal1, Cheolju Lee2
1Center for Theragnosis, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea; Division of Bio-Medical Science & Technology, KIST School, Korea University of Science and Technology, Seoul 02792, Republic of Korea.
Journal of Proteomics
|December 28, 2020
Summary
N-terminomics, the study of protein N-terminal sequences, has advanced significantly. This review details evolving methods, applications in understanding biological pathways, and potential for identifying disease biomarkers and drug targets.
Area of Science:
- Proteomics
- Biochemistry
- Molecular Biology
Background:
- N-terminomics studies protein N-terminal sequences, crucial for understanding proteolytic cascades and genome annotation.
- Post-translational modifications (PTMs) at protein termini impact biological pathways.
- Advancements in mass spectrometry have enhanced N-terminomic capabilities.
Purpose of the Study:
- To review the evolution of N-terminomic strategies and their applications.
- To compare different N-terminomic methods, highlighting benefits and limitations.
- To provide an overview of bioinformatics tools for N-terminomic data analysis.
Main Methods:
- Discussion of various sample preparation and N-terminal peptide enrichment strategies.
- Comparative analysis of different N-terminomic methodologies.
- Overview of bioinformatics tools and data analysis pipelines.
Main Results:
- N-terminomics provides a proteome-wide landscape of mature N-terminal sequences.
- It aids in annotating the source of protein generation and their biological significance.
- Improved methodologies enable the study of proteoforms and their clinical implications.
Conclusions:
- N-terminomics is vital for studying proteolytic machinery and identifying therapeutic targets.
- It facilitates the detection of low-abundance proteoforms and non-canonical protein sites.
- Applications include biomarker discovery and novel drug target identification.
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