Simultaneous quantification of ethylene glycol and diethylene glycol in beer by gas chromatography coupled to mass spectrometry
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Updated: Nov 24, 2025

Metabolomic Analysis of Barley by Gas Chromatography/Mass Spectrometry
Published on: November 8, 2024
Leila Rodrigues Caldeira1, Fernando Diniz Madureira2, Thalita De Faria Maia2
1Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil; Laboratório Federal de Defesa Agropecuária, Avenida Raja Gabaglia, 245, Setor H, Bairro Cidade Jardim, Belo Horizonte, MG 30380-103, Brazil.
Abstract:
An analytical method for the simultaneous determination of ethylene glycol and diethylene glycol in beer was developed and validated according to current legislation. This method includes the application of sample dilution with ethanol followed by quantification using gas chromatography coupled to mass spectrometry. All figures of merit were within the limits established by regulation. The recoveries of the analytes, expressed as mean recovery, were between 91.9% and 108.9%. Precision, in terms of repeatability and intermediate precision, was established (relative standard deviations were lower than or equal to 10%). The limits of detection (10.0 and 5.0 mg.L-1 for ethylene glycol and diethylene glycol, respectively) and quantification (15.0 mg.L-1 for ethylene glycol and diethylene glycol) obtained were appropriate. Finally, the present method was applied for determination of ethylene glycol and diethylene glycol in 701 beer samples (from 67 different brands and 128 different labels), proving to be reliable.
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