Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Retinoblastoma Gene01:20

The Retinoblastoma Gene

4.4K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.4K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

5.5K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
5.5K
Abnormal Proliferation02:23

Abnormal Proliferation

4.9K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.9K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

8.7K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.7K
Karyotyping01:17

Karyotyping

66.4K
Overview
66.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Arc of Riolan artery may serve as the only pathway for lymphatic metastasis in advanced splenic flexure cancer.

Techniques in coloproctology·2026
Same author

Raman spectroscopic signatures of amyloid fibrils: Insights into structural and biochemical changes in human tissues.

Biophysical chemistry·2025
Same author

Supramolecular arrangements in human amyloid tissues using SAXS.

Biophysical chemistry·2024
Same author

Spectroscopic diagnosis and metabolite characterization of cisplatin resistance regulated by FDFT1 in bladder cancer tissue.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2024
Same author

Epithelial-mesenchymal transition profiles in triple negative breast carcinoma may explain its aggressive nature.

The Malaysian journal of pathology·2023
Same author

Gastrointestinal stromal tumour in a jejunal diverticulum: The eighth reported case worldwide with a brief review of the literature.

The Malaysian journal of pathology·2023

Related Experiment Video

Updated: Nov 24, 2025

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations
08:22

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations

Published on: December 1, 2017

8.9K

FISHing for 1p19q codel in oligodendroglioma.

P L Kong1, P L Cheah, K S Mun

  • 1Genomic Medical Science, University Malaya, Faculty of Medicine and Health Sciences, Department of Pathology, Kuala Lumpur, Malaysia. po_lian88@siswa.um.edu.my.

The Malaysian Journal of Pathology
|December 28, 2020
PubMed
Summary

Establishing reference ranges for 1p19q co-deletion testing is crucial for oligodendroglioma diagnosis. This study defines new cut-offs using fluorescence in situ hybridization, improving diagnostic accuracy for this brain tumor.

More Related Videos

A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma DIPG
08:46

A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma DIPG

Published on: March 7, 2017

17.3K
Author Spotlight: Studying Clinical Characters and Epilepsy Outcomes After Frontal Disconnection in Patients with MOGHE
06:04

Author Spotlight: Studying Clinical Characters and Epilepsy Outcomes After Frontal Disconnection in Patients with MOGHE

Published on: August 16, 2024

1.3K

Related Experiment Videos

Last Updated: Nov 24, 2025

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations
08:22

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations

Published on: December 1, 2017

8.9K
A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma DIPG
08:46

A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma DIPG

Published on: March 7, 2017

17.3K
Author Spotlight: Studying Clinical Characters and Epilepsy Outcomes After Frontal Disconnection in Patients with MOGHE
06:04

Author Spotlight: Studying Clinical Characters and Epilepsy Outcomes After Frontal Disconnection in Patients with MOGHE

Published on: August 16, 2024

1.3K

Area of Science:

  • Neuro-oncology
  • Molecular Pathology
  • Genetics

Background:

  • Co-deletion of 1p19q (1p19q codel) is essential for diagnosing oligodendroglioma, alongside isocitrate dehydrogenase (IDH) mutation.
  • Histopathology labs need reliable testing for 1p19q codel, but consensus reference ranges and cut-offs are lacking.

Purpose of the Study:

  • To determine reference ranges for 1p and 19q deletion in non-neoplastic brain tissue.
  • To validate a fluorescence in situ hybridization (FISH) methodology for 1p19q codel testing in oligodendrogliomas.
  • To establish diagnostic cut-offs for 1p and 19q deletion.

Main Methods:

  • Utilized fluorescence in situ hybridization (FISH) with specific Vysis FISH Probe Kits.
  • Analyzed 11 formalin-fixed, paraffin-embedded non-neoplastic brain tissues to establish reference ranges.
  • Validated the methodology in 13 IDH-mutant oligodendrogliomas, assessing percentage of cells with deletion and target:control ratios.

Main Results:

  • Significant differences (p<0.000) were observed in 1p and 19q deletion percentages and target:control ratios between non-neoplastic brain tissue and oligodendrogliomas.
  • Established cut-offs for 1p deletion: >32-33% cells with deletion and/or target:control ratio <0.83.
  • Established cut-offs for 19q deletion: >22% cells with deletion and target:control ratio <0.88.

Conclusions:

  • The defined cut-offs accurately identified 1p19q codel in all tested oligodendrogliomas.
  • This study provides validated reference ranges and diagnostic cut-offs for 1p19q codel testing.
  • Implementation of these FISH-based cut-offs can enhance the diagnostic accuracy of oligodendroglioma.