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Differential phase imaging in full-field optical coherence microscopy using a short multimode fiber probe.

Manabu Sato, Junpei Masuta, Izumi Nishidate

    Applied Optics
    |December 28, 2020
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    This study introduces a novel phase imaging technique using a short multimode fiber (SMMF) probe to reduce phase noise in optical coherence microscopy. This method enhances imaging quality by providing clearer phase data for biological and material samples.

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    Area of Science:

    • Biomedical Optics
    • Microscopy Techniques
    • Phase Imaging

    Background:

    • Full-field optical coherence microscopy (FF-OCM) is a powerful imaging technique.
    • Phase noise is a common artifact that degrades FF-OCM image quality.
    • Short multimode fiber (SMMF) probes offer potential for miniaturized microscopy but can introduce phase instability.

    Purpose of the Study:

    • To develop and demonstrate a phase imaging method that reduces common phase noise in FF-OCM.
    • To utilize a SMMF probe for simultaneous phase measurement of the probe and sample.
    • To improve the accuracy and stability of phase measurements in FF-OCM.

    Main Methods:

    • Simultaneous phase imaging of a SMMF probe surface and a sample surface using a cover glass.
    • Employing the SMMF surface phase as a reference to subtract phase drifts from the sample region.
    • Quantitative evaluation of phase stability and resolution.

    Main Results:

    • Phase drifts in the sample region were significantly reduced by subtracting the SMMF surface phase.
    • The standard deviation of phase variation decreased from 14.3 to 9.2 degrees, a 64% reduction.
    • Achieved axial resolution of 2.3 µm and lateral resolution of <4.4 µm.
    • Measured phases closely corresponded to phase changes induced by a piezoelectric device.

    Conclusions:

    • The developed phase imaging technique effectively reduces phase noise in FF-OCM when using a SMMF probe.
    • Simultaneous reference phase measurement is a viable strategy for stabilizing FF-OCM.
    • This method enhances the reliability and quantitative accuracy of phase imaging for various applications.