Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Extracellular vesicles in fatty liver promote a metastatic tumor microenvironment.

Cell metabolism·2026

Same author

A RIPK2 activity signature in prostate cancer: Modulation by RIPK2 inhibition and clinical association.

Translational oncology·2026

Same author

Leveraging Quantitative Proteomics and Extracellular Vesicle Data to Uncover Druggable Receptor Kinases Across Cancers.

Journal of extracellular vesicles·2026

Same author

Noninvasive Assessment of Protease Activity in Osteosarcoma via Click Chemistry-Mediated Enrichment of Extracellular Vesicles.

Advanced functional materials·2026

Same author

Dynamic Targetable Extracellular Vesicle Surface Proteins Monitor Depth of Response to CAR T Therapy.

Research square·2026

Same author

Identification of Lupus Immune Complex-Driven Pathogenic Pro-inflammatory Monocytes and Macrophages in Systemic Lupus Erythematosus.

bioRxiv : the preprint server for biology·2026

Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026

Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026

Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026

Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026

Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026

Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 24, 2025

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Microscope calibration protocol for single-molecule microscopy.

Sungyong You, Jerry Chao, Edward A K Cohen

|December 28, 2020

Summary

This summary is machine-generated.

Accurate microscope calibration is crucial for single-molecule imaging. This study introduces a nanometer-scale calibration method to precisely measure optical aberrations, enhancing multicolor imaging accuracy.

More Related Videos

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

Published on: July 5, 2021

Related Experiment Videos

Last Updated: Nov 24, 2025

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

Published on: July 5, 2021

Area of Science:

Biophysics
Optical Microscopy
Nanotechnology

Background:

Single-molecule microscopy offers high-resolution insights into molecular dynamics and subcellular structures.
Accurate calibration is essential for high-fidelity multicolor single-molecule imaging.
Existing calibration methods may lack the precision required for nanometer-scale optical aberration analysis.

Purpose of the Study:

To propose and validate a novel method for nanometer-scale microscope calibration.
To quantify optical aberrations by analyzing point source localization errors.
To provide calibration procedures specifically supporting multicolor imaging, including dichroic filter and objective lens aberrations.

Main Methods:

Imaging of a standard sample to detect geometric aberrations in the optical path.
Analysis of point source localization errors to determine nanometer-scale aberration levels.
Development of specific protocols for evaluating dichroic filter-induced aberrations and objective lens axial chromatic aberration.

Main Results:

Demonstrated a method for nanometer-scale calibration of microscopes.
Quantified geometric aberrations affecting optical light paths.
Provided procedures for assessing aberrations critical for multicolor imaging, such as those from dichroic filters and objective lenses.

Conclusions:

The proposed method enables accurate nanometer-scale calibration of microscopes for single-molecule imaging.
This approach improves the reliability of multicolor imaging by precisely characterizing optical aberrations.
The findings contribute to advancing high-resolution microscopy techniques.