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Sural Nerve Perfusion in Mice.

Anete Dudele1,2, Peter Mondrup Rasmussen1, Leif Østergaard1,2,3

  • 1Center of Functionally Integrative Neuroscience (CFIN), Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.

Frontiers in Neuroscience
|December 28, 2020
PubMed
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This study introduces a new method using two-photon microscopy to measure blood flow in mouse peripheral nerves. The findings reveal insights into nerve hemodynamics and temperature sensitivity, crucial for understanding neuropathy.

Area of Science:

  • Neuroscience
  • Physiology
  • Biomedical Engineering

Background:

  • Peripheral nerve function requires significant energy, and impaired nerve energy metabolism is linked to diabetic neuropathy and neuropathic pain.
  • Assessing oxygen supply in distal peripheral nerves is difficult due to reliance on limited, nearby capillary vessels.

Purpose of the Study:

  • To develop and validate an in vivo method for characterizing distal sural nerve hemodynamics in mice.
  • To investigate the impact of local temperature on nerve blood flow and red blood cell (RBC) velocity.

Main Methods:

  • Utilized in vivo two-photon laser scanning microscopy in anesthetized adult male mice.
  • Measured red blood cell (RBC) velocities and flux in sural nerve vessels.
  • Analyzed RBC velocity fluctuations using power spectral density analysis.
Keywords:
hindlimb temperaturemicenerve blood flowred blood cell velocitysural nervetwo-photon microscopy

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Main Results:

  • RBC velocities in mouse sural nerve vessels were higher than those in the brain and sensitive to hindlimb temperature.
  • Nerve blood flow (RBC flux) was comparable to the brain and not affected by local temperature.
  • The technique demonstrated sensitivity to detect subtle flow oscillations over various time scales.

Conclusions:

  • In vivo two-photon laser scanning microscopy is a viable method for studying peripheral nerve hemodynamics in mice.
  • Local temperature control is critical for accurate measurements of peripheral nerve blood flow.