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Updated: Nov 24, 2025
Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies
Published on: March 2, 2018
Circulating Neutrophil-Derived Microparticles Associated with the Prognosis of Patients with Sepsis
Hong-Peng Chen1,2, Xiao-Yan Wang1, Xiao-Yan Pan1
1Department of Critical Care Medicine, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, People's Republic of China.
Introduction:
Because of its high morbidity and mortality, sepsis remains the leading cause of death in the ICU. Microparticles (MP) have been largely studied as potential diagnostic or prognostic markers in various diseases including sepsis.
Objective:
The biological and clinical relevance of neutrophil-derived microparticles (NDMPs) within the MP population remains unclear. The objective of this study was to elucidate the relationship between plasma NDMPs and the prognosis of patients with sepsis and/or septic shock.
Methods:
The study was designed as an observational, noninterventional clinical study. The cohort for this study included 40 sepsis and 40 septic shock patients together with 10 healthy controls admitted to the Intensive Care Unit (ICU) and the Health Surveillance Center in the Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China, from January to November 2018, respectively. The degree of critical disease for sepsis and septic shock was evaluated, with data analyses conducted from 2018 to 2019.
Results:
On days 1, 3 and 5 post-admission a series of data including plasma NDMP levels, patient demographics, TNF-α levels, IL-6 levels, sTREM-1 levels, and the sepsis severity score measurements were collected. A survival curve was plotted against levels of plasma NDMPs. Levels of NDMPs were observed to be higher in the septic shock patients than in the sepsis patients on days 1, 3, and 5 post-ICU admission (p < 0.05). NDMP levels were significantly increased in sepsis and septic shock patients with a parallel increase in pro-inflammatory mediators and sepsis severity score (p < 0.05) as well as mortality.
Conclusion:
Our data suggest that NDMPs may be a biomarker of sepsis severity and mortality although its implications on sepsis prognosis warrant further study.
Insights
Neutrophil-derived microparticles (NDMPs) were higher in sepsis and septic shock patients, correlating with increased severity and mortality. These findings suggest NDMPs may serve as a biomarker for sepsis prognosis.
Area of Science:
- Critical Care Medicine
- Immunology
- Biomarker Discovery
Background:
- Sepsis is a leading cause of ICU mortality, necessitating improved diagnostic and prognostic markers.
- Microparticles (MPs) are studied for their potential in disease prognostication, including sepsis.
- The specific role of neutrophil-derived microparticles (NDMPs) in sepsis prognosis is not well-defined.
Purpose of the Study:
- To investigate the relationship between plasma NDMP levels and the prognosis of patients with sepsis and/or septic shock.
- To determine if NDMPs can serve as a biomarker for sepsis severity and mortality.
Main Methods:
- Observational, noninterventional clinical study.
- Included 40 sepsis patients, 40 septic shock patients, and 10 healthy controls.
- Collected plasma NDMP levels, inflammatory mediators (TNF-α, IL-6), and sepsis severity scores on days 1, 3, and 5 post-ICU admission.
Main Results:
- Plasma NDMP levels were significantly higher in septic shock patients compared to sepsis patients on days 1, 3, and 5.
- Elevated NDMP levels correlated with increased pro-inflammatory mediators and sepsis severity scores.
- Higher NDMP levels were associated with increased patient mortality.
Conclusions:
- Neutrophil-derived microparticles (NDMPs) may indicate sepsis severity and predict mortality.
- NDMPs show potential as a biomarker for sepsis prognosis.
- Further research is needed to fully understand the implications of NDMPs in sepsis prognosis.
