Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

3.1K
Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of a recombinant single-cycle influenza viral vector as an intranasal vaccine against SARS-CoV-2.

Scientific reports·2026
Same author

Reactive astrocytes mediate toxicity in iPSC derived dopaminergic neurons.

NPJ Parkinson's disease·2026
Same author

Single-cell and spatial transcriptomic analyses of gene therapy-associated retinal inflammation in non-human primates.

Molecular therapy. Advances·2026
Same author

Tracking tau and cellular responses in human iPSC-microglia: from uptake to seedable secretion, including in extracellular vesicles.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same author

Basic Science and Pathogenesis.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

Development of a Recombinant Single-Cycle Influenza Viral Vector as an Intranasal Vaccine against SARS-CoV-2 and SARS-like Betacoronaviruses.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Nov 24, 2025

Author Spotlight: Induced Microglia-Like Cell Technology to Shed Light on the Role of Microglial Dysfunction in Neuropsychiatric Disorders
06:12

Author Spotlight: Induced Microglia-Like Cell Technology to Shed Light on the Role of Microglial Dysfunction in Neuropsychiatric Disorders

Published on: September 6, 2024

1.7K

Honing the Double-Edged Sword: Improving Human iPSC-Microglia Models.

Anne Hedegaard1, Szymon Stodolak1, William S James1

  • 1Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.

Frontiers in Immunology
|December 28, 2020
PubMed
Summary

Human induced pluripotent stem cell (hiPSC)-derived microglia offer insights into neuroinflammation. Current models face challenges in balancing physiological relevance, reproducibility, and scalability for neurodegenerative disease research.

Keywords:
3D scaffoldsco-culturehumanin vitro modelsinduced pluripotent stem cellsmedia compositionmicrogliaphysiological relevance

More Related Videos

Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route
05:35

Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route

Published on: May 31, 2022

3.2K
Human Microglia-like Cells: Differentiation from Induced Pluripotent Stem Cells and In Vitro Live-cell Phagocytosis Assay using Human Synaptosomes
11:19

Human Microglia-like Cells: Differentiation from Induced Pluripotent Stem Cells and In Vitro Live-cell Phagocytosis Assay using Human Synaptosomes

Published on: August 18, 2022

4.6K

Related Experiment Videos

Last Updated: Nov 24, 2025

Author Spotlight: Induced Microglia-Like Cell Technology to Shed Light on the Role of Microglial Dysfunction in Neuropsychiatric Disorders
06:12

Author Spotlight: Induced Microglia-Like Cell Technology to Shed Light on the Role of Microglial Dysfunction in Neuropsychiatric Disorders

Published on: September 6, 2024

1.7K
Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route
05:35

Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route

Published on: May 31, 2022

3.2K
Human Microglia-like Cells: Differentiation from Induced Pluripotent Stem Cells and In Vitro Live-cell Phagocytosis Assay using Human Synaptosomes
11:19

Human Microglia-like Cells: Differentiation from Induced Pluripotent Stem Cells and In Vitro Live-cell Phagocytosis Assay using Human Synaptosomes

Published on: August 18, 2022

4.6K

Area of Science:

  • Neuroscience
  • Stem Cell Biology
  • Immunology

Background:

  • Neuroinflammation is a critical factor in neurodegenerative diseases and aging.
  • Microglia are key immune cells involved in neuroinflammation.
  • Human induced pluripotent stem cell (hiPSC)-derived microglia are emerging as a powerful research tool.

Purpose of the Study:

  • To evaluate current hiPSC-derived microglial models for studying neuroinflammation.
  • To identify limitations in existing models regarding physiological relevance, reproducibility, and scalability.
  • To pinpoint areas for improvement in the *in vitro* microglial environment.

Main Methods:

  • Analysis of key features in the *in vitro* microglial environment.
  • Examination of media composition, extracellular matrix, and co-culture systems.
  • Review of existing hiPSC-microglia model methodologies.

Main Results:

  • A variety of hiPSC-microglia models exist, from monocultures to xenotransplantation.
  • Achieving a balance of physiological relevance, reproducibility, and scalability remains a significant challenge.
  • Specific *in vitro* environmental factors require optimization for enhanced model utility.

Conclusions:

  • Optimizing media composition, extracellular matrix, and co-culture strategies is crucial for advancing hiPSC-microglia models.
  • Improved *in vitro* models will enhance the study of neuroinflammation in neurodegenerative diseases.
  • Further development is needed to create more robust and translatable hiPSC-microglia systems.