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Transcriptome Profiling Provides Insights Into Potential Antagonistic Mechanisms Involved in Chaetomium globosum
K Darshan1, Rashmi Aggarwal1, Bishnu Maya Bashyal1
1Fungal Molecular Biology Laboratory, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, India.
Frontiers in Microbiology
|December 28, 2020
Summary
This study reveals the molecular mechanisms of Chaetomium globosum biocontrol against wheat spot blotch. RNA-sequencing identified key genes involved in hydrolytic activity and secondary metabolite production, offering insights into fungal antagonism.
Area of Science:
- Plant Pathology
- Mycology
- Molecular Biology
- Biotechnology
Background:
- Chaetomium globosum is a potential biocontrol agent for wheat spot blotch caused by Bipolaris sorokiniana.
- The molecular mechanisms and biosynthetic pathways underlying C. globosum's biocontrol activity remain largely unknown.
Purpose of the Study:
- To elucidate the molecular mechanisms of antagonism employed by C. globosum against B. sorokiniana.
- To identify differentially expressed genes and pathways involved in the biocontrol interaction using global transcriptome profiling.
Main Methods:
- Global transcriptome profiling of C. globosum strain Cg2 during interaction with B. sorokiniana isolate BS112 using RNA-sequencing (RNA-seq).
- De novo assembly of transcriptomic data to generate unigenes and identify differentially expressed genes.
- Validation of RNA-seq data using RT-qPCR for selected genes.
Main Results:
- Transcriptome analysis identified 6,109 unique differentially expressed genes.
- Upregulated genes were predominantly involved in catalytic activity (45.06%), including hydrolytic activity (10.02%), and metabolic activity (29.18%).
- Increased expression of genes encoding secondary metabolites (e.g., polyketide synthase, terpene cyclase) and hydrolytic enzymes (e.g., glycosyl hydrolases, chitinases, β-1,3-glucanases, proteases) was observed.
Conclusions:
- This study provides the first global transcriptome analysis of the C. globosum-B. sorokiniana interaction, revealing key genes and pathways involved in biocontrol.
- The findings highlight the role of hydrolytic enzymes and secondary metabolites in the antagonistic mechanism of C. globosum.
- The generated dataset offers valuable insights for improving gene annotation models and advancing the application of C. globosum as a biocontrol agent.
