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Updated: Nov 24, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
Characterization and phylogenetic analysis of Corydoras arcuatus mitochondrial genome
Yi Liu1,2, Baohong Xu1,2, Tiaoyi Xiao1,2
1Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha, PR China.
Abstract:
Corydoras arcuatus is a kind of famous and precious ornamental fish. In this study, the complete mitochondrial genome yielded by next-generation sequencing of Corydoras arcuatus was assembled and analyzed. The total length of the mitochondrial genome is 16,822 bp, with the base composition of 32.40% A, 26.10% T, 26.30% C, and 15.20% G. It contains two ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes, and a major non-coding control region (D-loop region). The arrangement of these genes is the same as that found in the Siluriformes. All the protein initiation codons are ATG, except for cox1 that begins with GTG. The complete mitogenomes of C. arcuatus and other 17 species from nine genera were used for phylogenetic analysis by Neighbour-Joining method. The topology demonstrated that all species are divided into three groups (Siluridae, Loricariidae, and Callichthyidae), and the C. arcuatus was clustered with C. rabauti and C. nattereri.
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