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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
The plastid genome of a tropical tree Alseodaphne petiolaris (Lauraceae)
Rongjie Zhu1, Yu Song2, Guanfei Zhao1
1Institute of Vegetable Sciences, Tibet Academy of Agricultural and Animal Husbandry Sciences, Tibet, China.
Abstract:
Alseodaphne petiolaris Hook.f. is a valuable timber tree of the genus Alseodaphne Nees in the family Lauraceae. To better determine its phylogenetic location with respect to the other Alseodaphne species, the complete plastid genome of A. petiolaris was sequenced. The whole plastome is 152,986 bp in length, consisting of a pair of inverted repeat (IR) regions of 20,108 bp, one large single copy (LSC) region of 93,863 bp, and one small single copy (SSC) region of 18,907 bp. The overall GC content of the whole plastome is 39.1%. Further, maximum-likelihood phylogenetic analyse was conducted using 15 complete plastomes of the Lauraceae, which support the close relationship between A. petiolaris and the species of Machilus and Phoebe.
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