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Updated: Nov 24, 2025

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
Published on: July 28, 2017
The complete chloroplast genome sequence of Cynanchum forrestii Schltr. (Asclepiadaceae) and its phylogenetic
Jie Zhang1, Dequan Zhang1,2,3
1College of Pharmacy and Chemistry, Dali University, Dali, PR China.
Abstract:
Cynanchum forrestii is a folk medicinal plant in southwest China. In this study, we sequenced complete chloroplast (cp) genome sequence of C. forrestii to investigate its phylogenetic relationship. The whole cp genome of C. forrestii was 159,917 bp in length with 43.5% overall GC content, including a large single-copy (LSC) region of 91,189 bp and a small single-copy (SSC) region of 19,972 bp, which were separated by a pair of inverted repeats (IRs) of 24,378 bp. The cp genome contained 112 genes, including 78 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. The phylogenetic analysis based on cp genome sequences showed that Cynanchum was closely related with Asclepias and Calotropis.
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