Characterization of the complete mitochondrial genome and phylogenetic analysis of Prototheca stagnorum (chlorellales: Chlorellaceae)
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Updated: Nov 24, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
Qian Wu1, Yanhong Lan1, Hui Xu1
1Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China.
Abstract:
Prototheca stagnorum belongs to the genus Prototheca that are achloroplyllous algae with phylogenetic affinities to Chlorella sp. Microalgae of the genus Prototheca spp are associated with rare algal infections of invertebrates termed protothecosis. In this study, the complete nucleotide sequence of the circular mitochondrial (mt) DNA of the chlorophyte alga P. stagnorum has been determined (80,023 base-pairs, A + T content 13.77%). The genes identified encode three subunits of the cytochrome oxidase and apocytochrome b, eight subunits of the NADH dehydrogenase complex (nad1-7, nad4L), four ATPase subunits (atp1, atp4, atp6, atp8), three ribosomal RNAs (5 S (rrn5), small subunit (srn) and large subunit (lrn) RNA), 27 tRNAs, two succinate dehydrogenase and 10 ribosome proteins. The complete mitochondrial genome sequence will provide new molecular biology information to further understand the genetic diversity of the Prototheca sp. and to eliminate this population.
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