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Updated: Nov 24, 2025

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
Published on: July 28, 2017
Characterization of the complete chloroplast genome of Phleum pratense L. cv. Minshan
Guangxin Cui1, Yuan Lu1, Xiaoxing Wei2
1Lanzhou Institute of Husbandry and Pharmaceutical Science, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
Abstract:
Severe seed degradation of Phleum pratense L. cv. Minshan restricts its productivity and promotion, the chloroplast genome and evolutionary relationship analysis of Minshan could provide inheritance reasons on seed degradation and fundamental genetic reference for its molecular breeding and biological research. Its chloroplast genome was 134,973 bp in length, containing a pair of inverted repeated regions (42,726 bp) which were separated by a large single copy region of 79,473 bp, and a small single copy region of 12,774 bp. Moreover, a total of 114 functional genes were annotated, including 79 mRNA, 32 tRNA genes, and 5 rRNA genes. The phylogenetic relationships of 25 species indicated that Minshan was closely related to Avena damascene.
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