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Updated: Nov 24, 2025

Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
The complete chloroplast genome sequence of the threatened Cypripedium calceolus (Orchidaceae)
Li-Jie Zhang1, Rui Ding2, Wei-Wei Meng3
1College of Forestry, Shenyang Agricultural University, Shenyang, China.
Abstract:
The complete chloroplast genome of Cypripedium calceolus, a rare species in the family Orchidaceae was reported in this study. The genome size is 175,122 bp in length, and contains four sub-regions: 97,486 bp of large single copy (LSC) and 22,260 bp of small copy (SSC) regions, separated by 27,688 bp of inverted repeat (IR) regions. A total of 133 genes were annotated, including 87 protein-coding genes, 38 tRNA genes and 8 rRNA genes. The GC content of this cp genome is 34.36%. Phylogenetic analysis revealed a close relationship between C. calceolus with C. japonicum and C. formosanum. This is the first complete cp genome for C. calceolus that would be useful for conservation and phylogenetic studies of this species.
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