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Updated: Nov 24, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
The complete chloroplast genome of Liparis nervosa (Orchidaceae)
Qing-Hua Zhang1, Xiao-Ting Wang1, Shu-Zhao Zheng1
1Department of Forest Protection, Forestry College of Fujian Agriculture and Forestry University, Fuzhou, China.
Abstract:
Liparis nervosa, a terrestrial orchid was widely used as a traditional medicinal plant in China. In this study, we assembled the complete chloroplast genome of L. nervosa using Illumina sequencing data. The whole genome is 158,716 bp, contains a large single-copy region (LSC 86,010 bp), a small single-copy region (SSC 18,276 bp), and a pair of inverted repeats (IR 27,215 bp). The complete genome has 132 genes, including 77 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. In addition, a maximum-likelihood phylogenetic analysis demonstrated that L. nervosa was most closely related to Oberonia japonica. This work provides a theoretical basis for the development of conservation strategies of L. nervosa.
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