Related Experiment Video
Updated: Nov 24, 2025

Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
Single-molecule sequencing yields the complete chloroplast genome sequence of Populus deltoides
Dongyang Wu1, Yupeng Wang1, Donghua Wu2
1College of Information Science and Technology, Nanjing Forestry University, Nanjing, Jiangsu, China.
Abstract:
Populus deltoides is a fast-growing, large tree and one of the largest North American hardwood trees. In this study, the complete chloroplast (cp) genome sequence of P. deltoides is characterized. The whole cp genome was assembled to 156,867 bp, including a large single copy (LSC) region of 85,534 bp, a small single copy (SSC) region of 16,513 bp and a pair of inverted repeats (IRs) region of 27,410 bp. The base content of the P. deltoides cp genome is A (32.0%), T (31.3%), C (18.0%), and G (18.7%), and AT bases occupy a large proportion of the cp genome. The neighbor-joining phylogenetic analysis with 20 cp genomes from the Salicaceae family showed that P. deltoides is sister to Populus davidiana. These will provide for the evolutionary and biological studies in Salicaceae family.
More Related Videos
12:33Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
Published on: July 28, 2017
12:05A Workflow for the Quantitative Assessment of the Endophytic and Epiphytic Bacterial Microbiomes of the Bark of Populus trichocarpa
Published on: June 27, 2025
Related Concept Videos
Sanger Sequencing
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Evolutionary Relationships through Genome Comparisons
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Modern Molecular Taxonomy