Related Experiment Video
Updated: Nov 24, 2025
Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro
Published on: March 18, 2014
Cytotoxic and Apoptotic Effect of Nanoclinoptilolite on Canine Osteosarcoma Cell Lines
Pınar Alkım Ulutaş1, Funda Kıral1, Bülent Ulutaş2
1Department of Biochemistry, Aydin, Turkey.
Introduction:
Clinoptilolite has antiviral, antibacterial, anti-inflammatory, antidiabetic, and anticancer properties due to its biological activities. In various cancer cell culture studies, it has been reported effective against tumour cells and gave positive results in treatment of various tumours in dogs. No study was found on the effects of the nanoparticulate form, nanoclinoptilolite, on cancer cells. The aim of this study was to determine its cytotoxic and apoptotic effects in canine osteosarcoma (OSA) cell culture.
Material And Methods:
Doses at 50% inhibitory concentration were determined by measuring the dose- and duration-dependent cytotoxicity of nanoclinoptilolite on canine D-17 osteosarcoma cells by methylthiazol tetrazolium (MTT) test for 24 h, 48 h, and 72 h. Murine caspase-3 and -7 activity and expression levels of the BAX and BCL2 genes were measured using RT-PCR to investigate the apoptotic effect.
Results:
Nanoclinoptilolite decreased cell viability and induced caspase-3- and -7-mediated apoptosis in treated canine OSA cells. Furthermore, its application to canine OSA cells downregulated the expression of BCL2 and upregulated the expression of proapoptotic BAX.
Conclusion:
Clinoptilolite, which was previously demonstrated to have anticancer properties, decreased cell viability effectively and rapidly and increased the apoptotic cell ratio in a novel use in nanoparticle form, exhibiting this effect by increasing the BAX/BCL2 ratio.
Insights
Nanoclinoptilolite, a nanoparticulate form of clinoptilolite, effectively reduced canine osteosarcoma cell viability and induced apoptosis. This novel application shows promise by altering the BAX/BCL2 gene expression ratio.
Area of Science:
- Nanomedicine
- Veterinary Oncology
- Biochemistry
Background:
- Clinoptilolite exhibits known anticancer properties, but its nanoparticulate form, nanoclinoptilolite, has not been studied for effects on cancer cells.
- Previous research indicates clinoptilolite's efficacy against various tumors in cell cultures and canine treatments.
Purpose of the Study:
- To investigate the cytotoxic and apoptotic effects of nanoclinoptilolite on canine osteosarcoma (OSA) cell cultures.
- To evaluate the potential of nanoclinoptilolite as a therapeutic agent for canine osteosarcoma.
Main Methods:
- Determined 50% inhibitory concentration (IC50) of nanoclinoptilolite on canine D-17 OSA cells using MTT assay over 24, 48, and 72 hours.
- Assessed apoptosis by measuring caspase-3 and -7 activity and BAX and BCL2 gene expression via RT-PCR.
Main Results:
- Nanoclinoptilolite significantly decreased cell viability in canine OSA cells.
- Induced apoptosis mediated by caspase-3 and -7 activation.
- Downregulated BCL2 and upregulated proapoptotic BAX gene expression, increasing the BAX/BCL2 ratio.
Conclusions:
- Nanoclinoptilolite demonstrates rapid and effective cytotoxic and apoptotic effects on canine osteosarcoma cells.
- The nanoparticulate form enhances the anticancer properties of clinoptilolite, primarily by modulating the BAX/BCL2 ratio.
- This study highlights nanoclinoptilolite as a potential therapeutic candidate for canine osteosarcoma.
