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Updated: Nov 24, 2025

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
Published on: January 14, 2016
Cryptochrome 1 Inhibits Shoot Branching by Repressing the Self-Activated Transciption Loop of PIF4 in Arabidopsis
Huawei Zhai1, Lu Xiong1, Hongyu Li1
1The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China.
Abstract:
Cryptochrome 1 (CRY1) is an important light receptor essential for de-etiolation of Arabidopsis seedlings. However, its function in regulating plant architecture remains unclear. Here, we show that mutation in CRY1 resulted in increased branching of Arabidopsis plants. To investigate the underlying mechanism, we analyzed the expression profiles of branching-related genes and found that the mRNA levels of Phytochrome Interaction Factor 4 (PIF4) and PIF5 are significantly increased in the cry1 mutant. Genetic analysis showed that the pif4 or pif4pif5 mutant is epistatic to the cry1 mutant, and overexpression of PIF4 conferred increased branching. Moreover, we demonstrated that PIF4 proteins physically associate with the G-box motif within the PIF4 promoter to form a self-activated transcriptional feedback loop, while CRY1 represses this process in response to blue light. Taken together, this study suggests that the CRY1-PIF4 module regulates gene expression via forming a regulatory loop and shoot branching in response to ambient light conditions.
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