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Stability Indicating Method for Polysorbate 80 in Protein Formulations.

Gregory K Webster¹, Jean C Chang¹, Julie L Heflin¹

¹Analytical Research and Development, AbbVie Inc., North Chicago, IL 60064 USA.

Journal of Chromatographic Science

|December 28, 2020

Summary

This summary is machine-generated.

Optimizing online solid-phase extraction (SPE) for polysorbates in protein formulations improves detection of oxidation. This enhanced method provides a stability-indicating profile for routine analysis of these common pharmaceutical excipients.

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Area of Science:

Analytical Chemistry
Pharmaceutical Sciences
Biochemistry

Background:

Polysorbates (Tween) are crucial nonionic surfactants in protein formulations, preventing adsorption and enhancing stability.
Their complex, heterogeneous nature and susceptibility to oxidation pose analytical challenges, especially the lack of a strong chromophore.
Current routine analysis using online SPE simplifies profiles but merges polymers, hindering degradation detection.

Purpose of the Study:

To develop and investigate an optimized online SPE method for polysorbates in protein formulations.
To establish a stability-indicating analytical method that avoids the complexity of reversed-phase profiles.
To enable routine testing for polysorbate degradation in pharmaceutical products.

Main Methods:

Development and optimization of an online solid-phase extraction (SPE) method.
Utilized an expanded step gradient approach within the online SPE.
Investigated the method's effectiveness using polysorbate 80 as a model.

Main Results:

The optimized online SPE method successfully simplified the complex polysorbate profile.
Further expansion of the step gradient yielded a profile that is indicative of polysorbate degradation.
The developed method is suitable for routine stability testing of protein formulations.

Conclusions:

An optimized online SPE method offers a viable solution for analyzing polysorbates in protein formulations.
This approach addresses the limitations of previous methods by providing a stability-indicating profile.
The technique enhances the ability to detect polysorbate oxidation and ensure protein formulation stability.