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Novel pH-Sensitive Microbial Rhodopsin from Sphingomonas paucimobilis
N Maliar1, I S Okhrimenko1, L E Petrovskaya2
1Moscow Institute of Physics and Technology, Dolgoprudnyi, Moscow oblast, Russia.
Abstract:
This work provides the first characteristics of the rhodopsin SpaR from Sphingomonas paucimobilis, aerobic bacteria associated with opportunistic infections. The sequence analysis of SpaR has shown that this protein has unusual DTS motif which has never reported in rhodopsins from Proteobacteria. We report that SpaR operates as an outward proton pump at low pH; however, proton pumping is almost absent at neutral and alkaline pH. The photocycle of this rhodopsin in detergent micelles slows down with an increase in pH because of longer Schiff base reprotonation. Our results show that the novel microbial ion transporter SpaR of interest both as an object for basic research of membrane proteins and as a promising optogenetic tool.
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