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Tissue factor-loaded collagen/alginate hydrogel beads as a hemostatic agent
Chengkun Liu1, Zhuang Shi1, Haiyan Sun1
1State Key Laboratory of Heavy Oil Processing, College of Chemical Engineering, China University of Petroleum (East China), Qingdao, China.
Journal of Biomedical Materials Research. Part B, Applied Biomaterials
|December 28, 2020
Summary
This study developed a novel hemostatic agent using tissue factor (TF) liposomes, collagen, and alginate. The composite hydrogel beads significantly accelerated blood coagulation and showed no cytotoxicity, offering a promising solution for hemorrhage control.
Area of Science:
- Biomaterials Science
- Biomedical Engineering
- Hemostasis Research
Background:
- Uncontrolled hemorrhage is a major cause of mortality globally.
- Bioinspired hemostatic composites offer a promising strategy to mitigate hemorrhage-related deaths.
- Existing hemostatic agents require enhancement to augment inherent hemostatic mechanisms.
Purpose of the Study:
- To develop an efficient hemostatic agent by combining tissue factor (TF) liposomes, collagen, and an alginate matrix.
- To create a composite hydrogel bead capable of augmenting multiple hemostatic pathways.
- To evaluate the hemostatic efficacy and safety of the developed composite.
Main Methods:
- Fabrication of TF, collagen, and alginate (TCA) composite hydrogel beads (2.5-3.5 mm diameter).
- Characterization using electron microscopy, infrared spectroscopy, rheology, and swelling tests.
- Assessment of TF-liposome release kinetics and procoagulant activity in rabbit blood.
- In vitro cytotoxicity evaluation using live/dead cell assays.
Main Results:
- TCA beads demonstrated controlled release of TF-liposomes, accelerated by temperature.
- TCA beads significantly reduced blood coagulation time to approximately 4.5 minutes compared to control (~14.4 minutes).
- A synergistic procoagulant effect was observed among TF, collagen, and alginate, with decreasing individual contributions.
Conclusions:
- The TCA hydrogel macrobeads exhibit potent hemostatic capabilities due to the synergistic action of their components.
- The developed composite demonstrates excellent biocompatibility with undetectable cytotoxicity.
- TCA hydrogel macrobeads represent a potential platform for developing advanced hemostatic agents to combat hemorrhage.
