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Comprehensive phenotyping of endothelial cells using flow cytometry 2: Human
Dillon Grant1, Nicholas Wanner1, Matthew Frimel1
1Department of Inflammation and Immunity, Cleveland Clinic, Cleveland, Ohio, USA.
Summary
This study details human-specific endothelial phenotypes, building on prior work. It reviews markers and methods for identifying endothelial cells in human vascular research.
Area of Science:
- Vascular Biology
- Immunology
- Cell Biology
Background:
- Clinical and animal samples are crucial for translating preclinical findings to human applications in vascular research.
- Part 1 of this series discussed general endothelial concepts and murine-specific phenotypes.
- Understanding human-specific endothelial phenotypes is essential for advancing translational vascular research.
Purpose of the Study:
- To provide a comprehensive overview of human-specific endothelial phenotypes.
- To detail pan-endothelial cell markers relevant to human studies.
- To review organ-specific endothelial antigens and immunophenotyping of blood-borne endothelial cells.
Main Methods:
- Review of literature on human endothelial cell markers.
- Analysis of organ-specific endothelial antigens.
- Description of flow cytometric immunophenotyping techniques for endothelial cells.
Main Results:
- Identification of key pan-endothelial cell markers in humans.
- Characterization of distinct organ-specific endothelial antigens.
- Outline of methods for analyzing circulating endothelial cells.
Conclusions:
- Human endothelial phenotypes exhibit unique characteristics distinct from animal models.
- Specific markers and immunophenotyping methods are vital for accurate identification of human endothelial cells.
- This review aids researchers in distinguishing human endothelial cell populations for translational studies.
