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Myricetin Apoptotic Effects on T47D Breast Cancer Cells is a P53-Independent Approach

Mitra Soleimani1, Nayereh Sajedi1

  • 1Department of Anatomical Sciences, Isfahan University of Medical Sciences, Iran.

Asian Pacific Journal of Cancer Prevention : APJCP
|December 28, 2020
PubMed
Abstract

Insights

Myricetin, a plant flavonoid, effectively induces apoptosis in T47D breast cancer cells. This natural compound activates both extrinsic and intrinsic cell death pathways, offering a potential therapeutic strategy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Natural Product Chemistry

Background:

  • Nutraceuticals, including flavonoids like myricetin, are explored for their role in cancer therapy, specifically in inducing cancer cell apoptosis.
  • Myricetin, a polyphenol found in various foods and beverages, has demonstrated apoptosis-inducing properties in preliminary studies.
  • Understanding the mechanisms by which myricetin affects cancer cells is crucial for its therapeutic application.

Purpose of the Study:

  • To investigate the apoptotic effects of myricetin on T47D breast cancer cells.
  • To elucidate the molecular mechanisms underlying myricetin-induced apoptosis in this cell line.

Main Methods:

  • T47D breast cancer cells were treated with myricetin at its IC50 concentration for 24, 48, and 72 hours.
  • Apoptosis was assessed using MTT assay, Annexin V assay, flow cytometry, caspase-3 assay, and Real-time PCR.
  • Gene expression analysis focused on apoptosis-related genes including caspases, BAX/Bcl-2 ratio, P53, BRCA1, and GADD45.

Main Results:

  • Myricetin treatment significantly increased the expression of apoptosis-related genes: caspase-3, caspase-8, and caspase-9.
  • The ratio of BAX/Bcl-2 was significantly altered, indicating a shift towards apoptosis.
  • Expression of P53, BRCA1, and GADD45 genes was significantly upregulated, confirmed by Annexin V assay and flow cytometry.

Conclusions:

  • Myricetin effectively enhances apoptosis in T47D breast cancer cells.
  • The compound activates both extrinsic and intrinsic apoptotic pathways.
  • Myricetin may exert its pro-apoptotic effects via the BRCA1-GADD45 pathway.
Keywords:
ApoptosisFlavonoidT47Dmyricetin

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