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Hazelnut Pollen Phenotyping Using Label-Free Impedance Flow Cytometry.

Lorenzo Ascari1, Valerio Cristofori2, Federico Macrì1

  • 1Department of Life Sciences and Systems Biology, University of Turin, Turin, Italy.

Frontiers in Plant Science
|December 28, 2020
PubMed
Summary
This summary is machine-generated.

Impedance flow cytometry (IFC) successfully characterized hazelnut pollen viability and sterility for the first time. This method revealed significant variation in pollen quality among cultivars, linked to chromosome translocations and hydration levels.

Keywords:
Corylus avellana L.cultivar selectionpollen hydrationpollen viabilityreciprocal chromosome translocationssterile pollen

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Area of Science:

  • Agricultural Science
  • Plant Biology
  • Cytogenetics

Background:

  • Hazelnut (Corylus avellana) exhibits sporophytic self-incompatibility and can experience high pollen sterility due to reciprocal chromosome translocations.
  • Understanding hazelnut pollen biology is crucial for agriculture and plant breeding, especially given its midwinter pollination under specific environmental conditions.

Purpose of the Study:

  • To apply impedance flow cytometry (IFC) for the first time to characterize hazelnut pollen biology, including viability and sterility.
  • To determine optimal pre-hydration conditions for accurate hazelnut pollen viability assessment.
  • To evaluate pollen viability and sterility across various hazelnut cultivars and wild genotypes.

Main Methods:

  • Impedance flow cytometry (IFC) was employed for label-free analysis of hazelnut pollen.
  • IFC was validated using dye exclusion microscopy.
  • Pollen hydration dynamics, viability, and sterility were assessed in 33 cultivars and two wild hazelnut accessions.

Main Results:

  • Pollen sterility rates varied significantly among hazelnut accessions, with distinct groups of highly sterile and good-quality pollen producers.
  • Optimal pollen hydration for viability assessment was achieved after approximately 6 hours of pre-hydration in humid chambers.
  • Pollen viability measurements were strongly influenced by hydration levels, highlighting the importance of humidity for pollen functionality.

Conclusions:

  • Impedance flow cytometry is a valuable tool for high-throughput phenotyping of hazelnut pollen.
  • Reciprocal chromosome translocations likely contribute to gametic semi-sterility observed in certain hazelnut cultivars.
  • Further research is needed to elucidate the causes of pollen sterility and its impact on hazelnut productivity.