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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

2.3K
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Types Of Column Chromatography01:29

Types Of Column Chromatography

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The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
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Analyte Adsorption and Distribution01:09

Analyte Adsorption and Distribution

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In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...
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Silica Gel Column Chromatography: Overview01:10

Silica Gel Column Chromatography: Overview

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Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
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Principles Of Column Chromatography01:13

Principles Of Column Chromatography

8.1K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

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Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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Recent trends in sorbents for bioaffinity chromatography.

Cigdem Kip1, Kadriye Özlem Hamaloğlu1, Cihan Demir1,2

  • 1Chemical Engineering Department, Hacettepe University, Ankara, Turkey.

Journal of Separation Science
|December 28, 2020
PubMed
Summary

Bioaffinity chromatography is a powerful technique for isolating biological molecules. This review summarizes recent advancements in supports, ligands, and methods for efficient purification in fields like proteomics and genomics.

Keywords:
boronate affinity chromatographycell affinity captureimmobilized metal affinity chromatographymetal oxide affinity chromatographyprotein A affinity chromatography

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Purification of biological molecules is essential for proteomics, genomics, and glycomics.
  • Bioaffinity chromatography offers efficient isolation through specific ligand-target interactions.
  • Advancements in purification techniques are crucial for complex biological sample analysis.

Purpose of the Study:

  • To review recent developments in bioaffinity chromatography over the past 5 years.
  • To summarize key properties of supports and immobilization techniques.
  • To provide an overview of binding agents and application methods.

Main Methods:

  • Literature review of bioaffinity chromatography advancements.
  • Analysis of support materials and immobilization strategies.
  • Summary of binding agent types and chromatographic methods.

Main Results:

  • Recent literature highlights significant progress in bioaffinity chromatography.
  • Properties of various support materials and immobilization techniques are detailed.
  • Diverse binding agents and application methodologies are discussed.

Conclusions:

  • Bioaffinity chromatography remains a vital technique for biological molecule purification.
  • Understanding recent developments enhances purification efficiency in omics fields.
  • This review consolidates current knowledge for researchers in the field.