Mechanochemical Regulation of Cell Adhesion by Incorporation of Synthetic Polymers to Plasma Membranes

Chemical control of cell-cell interactions using synthetic materials is useful for a wide range of biomedical applications. Herein, we report a method to regulate cell adhesion and dispersion by introducing repulsive forces to live cell membranes. To induce repulsion, we tethered amphiphilic polymers, such as cholesterol-modified poly(ethylene glycol) (PEG-CLS), to cell membranes. We found that the repulsive forces introduced by these tethered polymers induced cell detachment from a substrate and allowed cell dispersion in a suspension, modulated the speed of cell migration, and improved the separation of cells from tissues. Our analyses showed that coating the cells with tethered polymers most likely generated two distinct repulsive forces, lateral tension and steric repulsion, on the surface, which were tuned by altering the polymer size and density. We modeled how these two forces are generated in kinetically distinctive manners to explain the various responses of cells to the coating. Collectively, our observations demonstrate mechanochemical regulation of cell adhesion and dispersion by simply adding polymers to cells without genetic manipulation or chemical synthesis in the cells, which may contribute to the optimization of chemical coating strategies to regulate various types of cell-cell interacting systems.