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In Vitro Assay to Study Tumor-macrophage Interaction
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Tumor-associated macrophages based signaling pathway analysis and hub genes identification in glioma

Xiang Wang1, Weihai Ning, Zhiqiang Qiu

  • 1Department of Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, China.

Medicine
|December 29, 2020
PubMed

Insights

Tumor-associated macrophages (TAMs) are key in glioma immunity. This study identified signaling pathways and six hub genes (C3, IL6, ITGB2, PTAFR, TIMP1, VAMP8) linked to TAMs, offering potential therapeutic targets for glioma.

Area of Science:

  • Oncology
  • Immunology
  • Bioinformatics

Background:

  • Tumor-associated macrophages (TAMs) are vital in cancer immunity, but their signaling in the glioma microenvironment is unclear.
  • Understanding glioma-TAM interactions is crucial for developing new therapies.

Purpose of the Study:

  • To explore signaling pathways regulating TAMs in glioma.
  • To identify potential therapeutic targets for glioma by analyzing bioinformatics data.

Main Methods:

  • Utilized Chinese Glioma Genome Atlas (CGGA) and The Cancer Genome Atlas (TCGA) datasets.
  • Performed differential gene expression analysis, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, and protein-protein interaction (PPI) network analysis.

Main Results:

  • Confirmed the prognostic value of TAMs in glioma.
  • Identified 477 differentially expressed genes (DEGs) and key immune response pathways.
  • Discovered six hub genes (C3, IL6, ITGB2, PTAFR, TIMP1, VAMP8) positively correlated with CD68 expression and glioma prognosis.

Conclusions:

  • TAMs serve as significant prognostic indicators in glioma.
  • Uncovered underlying signaling pathways and identified six hub genes associated with TAMs in the glioma microenvironment.
  • These hub genes represent promising candidates for further investigation and therapeutic development in glioma.

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