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Related Concept Videos

Physical Methods for Controlling Microbial Growth: Temperature01:23

Physical Methods for Controlling Microbial Growth: Temperature

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Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
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Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
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Thermal Inactivation of Different Capripox Virus Isolates.

Janika Wolff1, Martin Beer1, Bernd Hoffmann1

  • 1Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany.

Microorganisms
|December 29, 2020
PubMed
Summary
This summary is machine-generated.

Heat treatment effectively inactivates Capripox viruses (CaPVs), a contagious livestock disease agent. A 1-hour incubation at 56°C is recommended for safe handling and transport of CaPVs in lower biosecurity settings.

Keywords:
GTPVLSDVSPPVcapripoxgoatpoxheat inactivationlumpy skin diseasesheeppoxthermal inactivation

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Area of Science:

  • Veterinary Virology
  • Infectious Disease Control

Background:

  • Capripox viruses (CaPVs) cause significant livestock diseases.
  • High biosecurity level (BSL 3) is required for CaPV work, necessitating inactivation methods for lower biosecurity levels.

Purpose of the Study:

  • To determine optimal time-temperature profiles for CaPV inactivation using heat treatment.
  • To establish a reliable inactivation protocol for CaPVs in aqueous solutions, with and without serum.

Main Methods:

  • Testing four distinct CaPV isolates under various heat treatment conditions.
  • Assessing viral inactivation in aqueous solutions with and without protective serum.

Main Results:

  • Complete inactivation of all tested CaPV isolates was achieved after 30 minutes at 56°C or 10 minutes at 60°C.
  • Heat treatment proved to be a robust and effective inactivation method.

Conclusions:

  • A recommended inactivation procedure of 1 hour at 56°C ensures fail-safe inactivation of CaPVs.
  • This protocol facilitates safe handling, shipment, and work with CaPVs in laboratories with biosecurity levels lower than BSL 3.