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Thermal Inactivation of Different Capripox Virus Isolates
Janika Wolff1, Martin Beer1, Bernd Hoffmann1
1Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany.
Microorganisms
|December 29, 2020
Summary
Heat treatment effectively inactivates Capripox viruses (CaPVs), a contagious livestock disease agent. A 1-hour incubation at 56°C is recommended for safe handling and transport of CaPVs in lower biosecurity settings.
Area of Science:
- Veterinary Virology
- Infectious Disease Control
Background:
- Capripox viruses (CaPVs) cause significant livestock diseases.
- High biosecurity level (BSL 3) is required for CaPV work, necessitating inactivation methods for lower biosecurity levels.
Purpose of the Study:
- To determine optimal time-temperature profiles for CaPV inactivation using heat treatment.
- To establish a reliable inactivation protocol for CaPVs in aqueous solutions, with and without serum.
Main Methods:
- Testing four distinct CaPV isolates under various heat treatment conditions.
- Assessing viral inactivation in aqueous solutions with and without protective serum.
Main Results:
- Complete inactivation of all tested CaPV isolates was achieved after 30 minutes at 56°C or 10 minutes at 60°C.
- Heat treatment proved to be a robust and effective inactivation method.
Conclusions:
- A recommended inactivation procedure of 1 hour at 56°C ensures fail-safe inactivation of CaPVs.
- This protocol facilitates safe handling, shipment, and work with CaPVs in laboratories with biosecurity levels lower than BSL 3.
