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Calcium diffusion through perineurium of frog sciatic nerve.

K C Wadhwani1, H Levitan, S I Rapoport

  • 1Laboratory of Neurosciences, National Institute on Aging, Bethesda, Maryland 20892.

The American Journal of Physiology
|January 1, 1988
PubMed
Summary
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The frog sciatic nerve perineurium regulates calcium and sucrose transport, showing higher permeability for efflux than influx. This tissue can stabilize endoneurial calcium levels during plasma fluctuations.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Physiology

Background:

  • The perineurium forms a critical barrier in peripheral nerves.
  • Understanding ion and molecule transport across the perineurium is vital for nerve function.

Purpose of the Study:

  • To quantify calcium and sucrose permeabilities across the frog sciatic nerve perineurium.
  • To investigate factors influencing these transport mechanisms.
  • To assess the perineurium's role in regulating endoneurial calcium.

Main Methods:

  • Flux measurements of 45Ca and [3H]sucrose across isolated and intact frog sciatic nerve segments.
  • Perfusion of everted and normal perineurial cylinder configurations.
  • Variations in bathing calcium concentration, sodium-free Ringer, and application of ouabain, La3+, and 2,4-dinitrophenol.

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Main Results:

  • Mean calcium permeability (PCa) for influx was 10.2 x 10(-7) cm/s and for efflux was 27.5 x 10(-7) cm/s.
  • Mean sucrose permeability (Psucrose) for influx was 7.4 x 10(-7) cm/s and for efflux was 23.2 x 10(-7) cm/s.
  • Calcium accumulation in perineurial tissue occurred in a saturable manner (Km = 80 microM, Bmax = 0.22 mumol/g wet wt).
  • No significant effects on permeabilities were observed with varied calcium concentrations or specific inhibitors.

Conclusions:

  • Asymmetrical fluxes suggest bulk flow and solvent drag influence transport.
  • The perineurium exhibits calcium-sequestering ability with a long exchange half-time (3 h).
  • The perineurium plays a role in stabilizing endoneurial calcium concentrations against transient plasma changes.