Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Intercellular communication between ciliated cells in culture.

M J Sanderson¹, I Chow, E R Dirksen

¹Department of Anatomy, University of California, School of Medicine, Los Angeles 90024.

The American Journal of Physiology

|January 1, 1988

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Frailty predicts morbidity, complications, and mortality in patients undergoing complex abdominal wall reconstruction.

Hernia : the journal of hernias and abdominal wall surgery·2019

Same author

Homology-Aware Phylogenomics at Gigabase Scales.

Systematic biology·2017

Same author

Sources of error and confidence intervals in estimating the age of angiosperms from rbcL and 18S rDNA data.

American journal of botany·2011

Same author

Reconstructing shifts in diversification rates on phylogenetic trees.

Trends in ecology & evolution·2011

Same author

Phylogenetic supertrees: Assembling the trees of life.

Trends in ecology & evolution·2011

Same author

A biomechanical model of agonist-initiated contraction in the asthmatic airway.

Respiratory physiology & neurobiology·2009

Same journal

Blood coagulation in fish.

The American journal of physiology·2011

Same journal

Renal tubular reabsorption, metabolic utilization and isomeric fractionation of lactic acid in the dog.

The American journal of physiology·2010

Same journal

The inactivation of placental toxin by human serum.

The American journal of physiology·2010

Same journal

Adrenal function following ovariectomy in the rat.

The American journal of physiology·2010

Same journal

Capillary permeability; perfusion of frog and guinea pig hind limbs.

The American journal of physiology·2010

Same journal

Evaluation of protective measures against sunburn.

The American journal of physiology·2010

See all related articles

Mechanical stimulation of respiratory ciliated cells triggers a rapid increase in beat frequency. This response spreads to neighboring cells via intercellular communication, facilitated by gap junctions, enhancing mucus transport.

Area of Science:

Cell Biology
Respiratory Physiology
Biophysics

Background:

Mammalian respiratory tract ciliated cells exhibit coordinated beating for mucus clearance.
Mechanical stimulation is known to affect ciliary beat frequency.
The precise mechanism and speed of intercellular communication in this response are not fully understood.

Purpose of the Study:

To characterize the spatial and temporal dynamics of the ciliary beat frequency response to mechanical stimulation.
To investigate the role of intercellular communication in propagating this response.
To determine the speed of signal transmission between ciliated cells.

Main Methods:

Development of an automated computer-assisted image-analysis system for high-speed video analysis.

Related Experiment Videos

Cultured mammalian respiratory ciliated cells were subjected to mechanical stimulation.

High-speed films of cell responses were analyzed to measure beat frequency changes and transmission speed.

Main Results:

Mechanical stimulation induced a rapid, transient increase in ciliary beat frequency.
The frequency response propagated to adjacent and distal cells with a delay proportional to distance.
Transmission speeds were measured at 0.63 cells/s at 25°C and 1.54 cells/s at 37°C.
Electrical coupling via gap junctions was confirmed between ciliated and non-ciliated cells.

Conclusions:

Mechanical stimulation and intercellular communication, mediated by gap junctions, regulate ciliary beat frequency.
This communication mechanism ensures coordinated ciliary function for efficient mucus transport.
The findings provide insights into the physiological regulation of airway clearance.