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Related Experiment Video

Updated: Nov 24, 2025

Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae
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Generating liver using blastocyst complementation: Opportunities and challenges.

Rajagopal N Aravalli1

  • 1Department of Electrical and Computer Engineering, College of Science and Engineering, University of Minnesota, Minneapolis, MN, USA.

Xenotransplantation
|December 29, 2020
PubMed
Summary
This summary is machine-generated.

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In vitro Differentiation of Human TERT-Transfected Multi-Lineage Progenitor Cells (MLPC) into Immortalized Hepatocyte-Like Cells.

Hepatic medicine : evidence and research·2020
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Precision Targeted Ablation of Fine Neurovascular Structures In Vivo Using Dual-mode Ultrasound Arrays.

Scientific reports·2020
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Hepatic Differentiation of Marmoset Embryonic Stem Cells and Functional Characterization of ESC-Derived Hepatocyte-Like Cells.

Hepatic medicine : evidence and research·2020
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CRISPR/Cas9 therapeutics for liver diseases.

Journal of cellular biochemistry·2017

Generating whole livers using blastocyst complementation could solve the donor shortage for liver transplantation. This regenerative medicine approach shows promise for creating xenogeneic organs for human use.

Area of Science:

  • Regenerative Medicine
  • Transplantation Biology
  • Bioengineering

Background:

  • Orthotopic liver transplantation (OLT) is the definitive treatment for end-stage liver disease.
  • The demand for donor livers significantly exceeds the available supply.
  • Current strategies aim to bridge the gap or bioengineer livers using hepatic cells.

Purpose of the Study:

  • To review the potential of blastocyst complementation for generating whole livers.
  • To explore the application of gene editing with blastocyst complementation for organogenesis.
  • To discuss the future perspectives of liver xenotransplantation in humans.

Main Methods:

  • Blastocyst complementation for generating xenogeneic organs in animal models.
  • Integration of gene editing technologies to enhance organ development.
Keywords:
Hhexblastocyst complementationchimeragene editingliverpigstem cellsxenotransplantation

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  • Review of current knowledge and experimental strategies in liver bioengineering.

    • Main Results:

    • Blastocyst complementation has successfully produced xenogeneic organs in animal hosts.
    • This method holds promise for creating whole livers in large animals for xenotransplantation.
    • Significant progress has been made in regenerative medicine through this approach.

    Conclusions:

    • Blastocyst complementation combined with gene editing offers a promising solution to the donor liver shortage.
    • Xenotransplantation of bioengineered livers could alleviate the critical need for OLT.
    • Further research and ethical considerations are necessary for clinical application in humans.