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Long noncoding RNA small nucleolar RNA host gene 15 deteriorates liver cancer via microRNA-18b-5p/LIM-only 4 axis

Wei Chen1, Li Huang2, Junhua Liang1

  • 1Department of Gastroenterology, Xiangyang No.1 People's Hospital, Hubei University of Medicine, Xiangyang, China.

IUBMB Life
|December 29, 2020
PubMed

Insights

This study reveals that long noncoding RNA SNHG15 promotes liver cancer by interacting with microRNA 18b-5p and LIM-only 4. Silencing SNHG15 inhibits liver cancer progression, suggesting a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) play roles in liver cancer, but the function of small nucleolar RNA host gene 15 (SNHG15) remains unclear.
  • Understanding lncRNA mechanisms is crucial for developing novel liver cancer therapies.

Purpose of the Study:

  • To investigate the role and mechanism of SNHG15 in liver cancer.
  • To explore the regulatory network involving SNHG15, microRNA-18b-5p (miR-18b-5p), and LIM-only 4 (LMO4) in liver cancer progression.

Main Methods:

  • Analysis of SNHG15 expression in liver cancer tissues and correlation with patient prognosis and clinicopathological features.
  • In vitro and in vivo experiments using SMMC-7721 liver cancer cells with SNHG15 knockdown or miR-18b-5p restoration.
  • Detection of SNHG15, miR-18b-5p, and LMO4 expression and investigation of their regulatory interactions.

Main Results:

  • SNHG15 and LMO4 were upregulated, while miR-18b-5p was downregulated in liver cancer tissues and cells.
  • High SNHG15 expression correlated with poor prognosis and aggressive tumor behavior.
  • SNHG15 knockdown or miR-18b-5p restoration suppressed liver cancer cell proliferation, migration, and invasion.
  • SNHG15 directly binds to miR-18b-5p, and miR-18b-5p targets LMO4.

Conclusions:

  • Silencing SNHG15 inhibits liver cancer progression by modulating the miR-18b-5p/LMO4 axis.
  • The SNHG15/miR-18b-5p/LMO4 pathway represents a potential therapeutic target for liver cancer treatment.
Keywords:
LIM-only 4liver cancerlong noncoding RNA small nucleolar RNA host gene 15metastasismicroRNA-18b-5pprognosis

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