[Effects of Adding Straw and Biochar with Equal Carbon Content on Soil Respiration and Microbial Biomass Carbon and...

In order to investigate the response of soil respiration, soil microbial biomass carbon and nitrogen, and hydrothermal factors to the addition of biochar and straw, we used an LI-8100 soil carbon flux meter (LI-COR, Lincoln, USA) to study changes in soil respiration and microbial biomass under four treatments:conventional fertilization (CK), conventional fertilization +2.25t·hm^-2 biochar-C (T1), conventional fertilizer +2.25t·hm^-2 straw-C (T2), and conventional fertilizer +2.25t·hm^-2 (biochar-C+straw-C), biochar-C:straw-C=1:1 (T3). The results showed that:① the addition of biochar and straw significantly increased the soil respiration rate and total CO₂ emissions, with the largest increase in T3 and the smallest increase in T1. The effect of T1 on soil respiration was promoted in the early stage and later inhibited. ② The microbial biomass carbon and nitrogen and the number of functional bacterial colonies increased significantly with biochar and straw amendments. T1 had a significant promotion effect on nitrogen-fixing bacteria, while T2 had no significant effect on the number of fungi, and T3 showed a positive interaction effect. Soil respiration rates were significantly and positively related to soil microbial biomass carbon and nitrogen as well as to the number of bacteria and actinomycetes. ③ The 5 cm soil temperature of T3 significantly increased by 4.53%. The soil respiration rate and soil temperature showed a significant exponential correlation. To sum up, adding straw and biochar with equal carbon content can significantly increase the soil respiration rate and microbial biomass, and the interaction effect between biochar and straw is positive. Compared with that of the straw treatments, the application of biochar can reduce carbon mineralization to a certain extent, and the effect of carbon sequestration is better.