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Diallyl trisulfide regulates cell apoptosis and invasion in human osteosarcoma U2OS cells through regulating

Pan He1, Zhijun Wang2, Bin Sheng2

  • 1The Department of Traumatic and Osteopathology, Hunan provincial people's hospital, Changsha, Hunan, China. h13487490749@126.com.

Histology and Histopathology
|December 29, 2020
PubMed
Abstract

Insights

Diallyl trisulfide (DATS) effectively inhibits osteosarcoma (OS) cell proliferation and metastasis by inducing apoptosis and regulating key signaling pathways. This natural compound shows promise as a potential therapeutic agent for OS treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Osteosarcoma (OS) is a primary bone malignancy with a high propensity for metastasis.
  • Effective therapeutic strategies for OS, particularly targeting proliferation and metastasis, are urgently needed.
  • Natural compounds are being explored for their potential anticancer properties.

Purpose of the Study:

  • To investigate the effects of Diallyl trisulfide (DATS) on the proliferation and metastasis of human osteosarcoma (OS) U2OS cells.
  • To elucidate the underlying mechanisms of action of DATS in OS.

Main Methods:

  • U2OS cells were treated with varying concentrations of DATS.
  • Cell proliferation was assessed using MTT assays.
  • Apoptosis and cell cycle distribution were analyzed by flow cytometry (FCM).
  • Migration and invasion were evaluated using wound healing and Transwell assays.
  • In vivo efficacy was tested in a mouse OS xenograft model.

Main Results:

  • DATS exhibited a time- and concentration-dependent cytotoxic effect on U2OS cells.
  • DATS induced apoptosis and G0/G1 cell cycle arrest.
  • DATS significantly inhibited cell migration and invasion in vitro.
  • In vivo studies showed reduced tumor volume, weight, and lung metastasis in DATS-treated mice.
  • DATS modulated the expression of key proteins involved in cell survival and metastasis, including the PI3K/AKT/GSK3β pathway.

Conclusions:

  • DATS effectively inhibits osteosarcoma progression by suppressing cell proliferation, migration, and invasion.
  • The anticancer effects of DATS are mediated through the regulation of PI3K/AKT/GSK3β signaling pathways.
  • DATS demonstrates potential as a therapeutic agent for osteosarcoma, either as a standalone treatment or in combination therapy.

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