Preventing Enamel Caries Using Carbon Dioxide Laser and Silver Diamine Fluoride

This study was intended to investigate the caries prevention potential of carbon dioxide (CO₂) laser (λ = 10,600 nm) irradiation followed by application of silver diamine fluoride (SDF) to enamel. Human enamel specimens were randomly allocated to four groups (n = 10 per group). Group 1 specimens were treated with SDF; Group 2 specimens were treated with a CO₂ laser; Group 3 specimens were irradiated with a CO₂ laser then treated with SDF, and Group 4 specimens received no treatment. All specimens were subjected to pH cycling for cariogenic challenge. Lesion depth, microhardness, surface morphology, and elemental analysis were assessed. The lesion depths for Groups 1-4 were 33 ± 16, 80 ± 9, 18 ± 15, and 102 ± 9 μm, respectively (p < 0.001; Group 3 < Group 1 < Group 2 < Group 4). Knoop hardness values for Groups 1-4 were 61 ± 19, 68 ± 20, 78 ± 27, and 36 ± 8, respectively (p = 0.002; Group 4 < Groups 1, 2, and 3). The enamel in Group 4 but not in the other groups showed a roughened surface resembling an acid-etched pattern. Calcium-to-phosphorus molar ratios of Groups 1-4 were 1.68 ± 60.09, 1.61 ± 0.06, 1.69 ± 0.10, and 1.49 ± 0.10, respectively (p < 0.001; Group 4 < Groups 1, 2, and 3). Using the CO₂ laser or SDF separately enhanced the resistance of enamel to cariogenic challenge. Moreover, there was an additional effect of the combined use of the CO₂ laser and SDF for preventing enamel demineralization.