Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

"We're not one-box issue people": Intersectional perspectives on LGBTQ+ mental health in schools: A UK qualitative study with students, staff and training providers.

Acta psychologica·2025
Same author

Intersectionality informed and narrative-shifting whole school approaches for LGBTQ+ secondary school student mental health: A UK qualitative study.

PloS one·2024
Same author

The incidence of melanoma in South Africa: An exploratory analysis of National Cancer Registry data from 2005 to 2013 with a specific focus on melanoma in black Africans.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2019
Same author

Circulating microRNA's as a diagnostic tool for hepatocellular carcinoma in a hyper endemic HIV setting, KwaZulu-Natal, South Africa: a case control study protocol focusing on viral etiology.

BMC cancer·2017
Same author

The management of pretibial lacerations.

Annals of the Royal College of Surgeons of England·2017
Same author

Prevalence of gastrointestinal parasitism in small ruminants in western zone of Punjab, India.

Veterinary world·2017
Same journal

Distinct cellular and subcellular distributions of the alpha and beta isoforms of Heat Shock Protein 90 in the mouse cerebral cortex.

Gene expression patterns : GEP·2026
Same journal

Molecular characterization and expression dynamics of Labeo rohita MDA5, its adaptor MAVS and pro-inflammatory cytokines against PAMPs stimulation and pathogen challenge.

Gene expression patterns : GEP·2026
Same journal

Defining the spatiotemporal expression pattern of Rap1 in the chicken embryonic spinal cord.

Gene expression patterns : GEP·2026
Same journal

Expression analysis of the TMC gene family in the auditory system during zebrafish embryonic development.

Gene expression patterns : GEP·2026
Same journal

Mapping the locations of barx1-expressing prechondrogenic condensation domains in the posterior arches during ceratobranchial cartilage formation in zebrafish.

Gene expression patterns : GEP·2026
Same journal

cDNA cloning, expression analysis, and subcellular localization of TRIM13 gene in zebrafish.

Gene expression patterns : GEP·2026
See all related articles

Related Experiment Video

Updated: Nov 23, 2025

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos
08:57

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

Published on: July 27, 2022

1.9K

Dynamic changes in endoglin expression in the developing mouse heart.

E Singh1, H M Phillips1, H M Arthur1

  • 1Biosciences Institute, Newcastle University, NE1 3BZ, UK.

Gene Expression Patterns : GEP
|December 29, 2020
PubMed
Summary
This summary is machine-generated.

Endoglin (ENG) protein expression changes dynamically during heart development. ENG is consistently found in veins but is downregulated in arteries and valve mesenchyme, indicating cell-type-specific roles.

Keywords:
CardiogenesisCoronary blood vesselsEndocardiumEndothelial cellsEpicardial derived cellsValve mesenchymal cells

More Related Videos

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart
06:51

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart

Published on: August 10, 2018

8.6K
Live Imaging of Early Cardiac Progenitors in the Mouse Embryo
07:02

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

Published on: July 12, 2022

1.7K

Related Experiment Videos

Last Updated: Nov 23, 2025

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos
08:57

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

Published on: July 27, 2022

1.9K
Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart
06:51

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart

Published on: August 10, 2018

8.6K
Live Imaging of Early Cardiac Progenitors in the Mouse Embryo
07:02

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

Published on: July 12, 2022

1.7K

Area of Science:

  • Cardiovascular Biology
  • Developmental Biology
  • Cell Biology

Background:

  • Endoglin (ENG) is crucial for cardiovascular development and present in the developing heart.
  • Previous studies noted ENG in various cardiac cell types, but its dynamic expression patterns remained uncharacterized.

Purpose of the Study:

  • To systematically investigate the dynamic changes in Endoglin (ENG) protein expression during mouse heart development.
  • To analyze ENG expression in different cardiac cell lineages, including endocardium, vascular endothelium, and mesenchymal cells.

Main Methods:

  • Immunofluorescent staining was used to track ENG protein expression.
  • Analysis was performed on mouse embryonic hearts (11.5–17.5 days), postnatal, and adult hearts.

Main Results:

  • ENG protein is consistently expressed in endocardial and venous endothelial cells throughout heart development.
  • ENG expression is downregulated twofold in developing arterial cells compared to venous cells, a ratio maintained postnatally.
  • ENG is twofold downregulated in endocardial-derived valve mesenchyme, with complete loss of CD31 expression.
  • Epicardially derived cells (EPDCs) express low levels of ENG, similar to valve mesenchyme.

Conclusions:

  • Endoglin (ENG) exhibits dynamic expression changes in various cardiovascular cell types during heart development.
  • ENG expression levels vary significantly between endothelial cells (veins vs. arteries) and during endothelial-to-mesenchymal transitions.
  • These findings highlight cell-type-specific roles of ENG in cardiac development.