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Characterization of cadmium-responsive transcription factors in wolf spider Pardosa pseudoannulata

Juan Wang1, Yuan He1, Xianjin Peng1

  • 1College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, China.

Chemosphere
|December 29, 2020
PubMed

Insights

Cadmium exposure negatively impacts spider growth and antioxidant enzyme activity. This study identifies key transcription factors (TFs) involved in the spider

Area of Science:

  • Environmental toxicology
  • Molecular biology
  • Spider biology

Background:

  • Transcription factors (TFs) regulate gene expression across all organisms.
  • Understanding TF roles is crucial for deciphering gene regulation mechanisms.
  • Cadmium (Cd) exposure can induce oxidative stress and affect growth in invertebrates.

Purpose of the Study:

  • To investigate the role of transcription factors in the molecular response of the spider Pardosa pseudoannulata to cadmium stress.
  • To identify candidate Cd-responsive TFs associated with growth and antioxidant defense in P. pseudoannulata.

Main Methods:

  • Transcriptome profiling of P. pseudoannulata under cadmium exposure.
  • Differential gene expression analysis to identify TFs.
  • Bioinformatic analysis to identify hub genes and conserved TF families.
  • Sequence analysis of candidate TFs (smads, foxo3) for conservation.

Main Results:

  • Cadmium exposure inhibited growth and increased antioxidant enzyme activity in P. pseudoannulata.
  • 1711 differentially expressed TFs were identified, with notable enrichment in zf-C2H2, ZBTB, Homeobox, and bHLH families.
  • Hub genes including smads, TCF7L2, EGR1, and GATA5 were identified as potential Cd-responsive TFs.
  • The TF foxo3 was found to regulate Sod2 expression, highlighting its role in antioxidant defense.
  • Conserved smads and foxo3 genes were identified in both spiders and insects.

Conclusions:

  • This study elucidates the critical role of transcription factors in the molecular response of P. pseudoannulata to cadmium stress for the first time.
  • Identified TFs provide insights into the mechanisms of cadmium toxicity and antioxidant defense in spiders.
  • Findings lay the groundwork for developing strategies to protect spider populations from cadmium contamination.
Keywords:
AntioxidantCadmiumGrowthPardosa pseudoannulataTranscription factor

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