Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

2.6K
Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
2.6K
Cell Migration01:19

Cell Migration

5.9K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
5.9K
Cell Migration01:09

Cell Migration

17.8K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
17.8K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

2.2K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.2K
Metastasis02:30

Metastasis

6.1K
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
6.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

N-cadherin facilitates trigeminal sensory neuron outgrowth and target tissue innervation.

Development (Cambridge, England)·2025
Same author

Inactivation of the SLC25A1 gene during embryogenesis induces a unique senescence program controlled by p53.

Cell death and differentiation·2024
Same author

N-cadherin facilitates trigeminal sensory neuron outgrowth and target tissue innervation.

bioRxiv : the preprint server for biology·2024
Same author

Loss of the mitochondrial carrier, <i>SLC25A1,</i> during embryogenesis induces a unique senescence program controlled by p53.

bioRxiv : the preprint server for biology·2023
Same author

Spontaneously evolved progenitor niches escape Yap oncogene addiction in advanced pancreatic ductal adenocarcinomas.

Nature communications·2023
Same author

Identifying drivers of breast cancer metastasis in progressively invasive subpopulations of zebrafish-xenografted MDA-MB-231.

Molecular biomedicine·2022
Same journal

RETRACTED: Kim et al. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Regulating the Visceral Adipose-Tissue Function. <i>Int. J. Mol. Sci.</i> 2017, <i>18</i>, 846.

International journal of molecular sciences·2026
Same journal

Correction: Mahmud et al. Thymoquinone Attenuates NF-κβ Signalling Activation in Retinal Pigment Epithelium Cells Under AMD-Mimicking Conditions. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 11473.

International journal of molecular sciences·2026
Same journal

Correction: Borovikov et al. The Twisting and Untwisting of Actin and Tropomyosin Filaments Are Involved in the Molecular Mechanisms of Muscle Contraction, and Their Disruption Can Result in Muscle Disorders. <i>Int. J. Mol. Sci</i>. 2025, <i>26</i>, 6705.

International journal of molecular sciences·2026
Same journal

Correction: Molagoda et al. Flavonoid Glycosides from <i>Ziziphus jujuba</i> var. <i>inermis</i> (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis. <i>Int. J. Mol. Sci.</i> 2021, <i>22</i>, 7701.

International journal of molecular sciences·2026
Same journal

Correction: Guo et al. Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots Under Lead Stress. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 6050.

International journal of molecular sciences·2026
Same journal

Correction: Chang et al. Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells Without Reprogramming Factor c-Myc. <i>Int. J. Mol. Sci.</i> 2012, <i>13</i>, 3598-3617.

International journal of molecular sciences·2026
See all related articles

Related Experiment Video

Updated: Nov 23, 2025

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60
08:13

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60

Published on: December 7, 2017

7.1K

miR614 Expression Enhances Breast Cancer Cell Motility.

Tuyen T Dang1,2, Alec T McIntosh3, Julio C Morales1

  • 1Department of Neurosurgery and Stephenson Cancer Center, University of Oklahoma Health Science Center, 1122 NE 13th St., Oklahoma City, OK 73117, USA.

International Journal of Molecular Sciences
|December 30, 2020
PubMed
Summary
This summary is machine-generated.

Increased miR614 expression enhances cell migration by upregulating Slug. Suppression of Miro1 or TAPT1 also boosts migration and Slug, with reduced TAPT1 linked to breast cancer relapse risk.

Keywords:
Miro1TAPT1miR614

More Related Videos

Studying TGF-&#946; Signaling and TGF-&#946;-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
06:54

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

13.8K
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
11:42

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

9.7K

Related Experiment Videos

Last Updated: Nov 23, 2025

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60
08:13

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60

Published on: December 7, 2017

7.1K
Studying TGF-&#946; Signaling and TGF-&#946;-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
06:54

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

13.8K
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
11:42

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

9.7K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • Epithelial-to-mesenchymal transition (EMT) is crucial for cell migration and metastasis.
  • MicroRNAs (miRNAs) play significant roles in regulating gene expression and cellular processes, including EMT.
  • Identifying novel regulators of EMT-induced cell migration is essential for understanding cancer progression.

Purpose of the Study:

  • To identify novel regulators of epithelial-to-mesenchymal transition (EMT) induced cell migration using a data-driven approach.
  • To investigate the role of miR614 in modulating cell migration and EMT.
  • To uncover specific genes regulated by miR614 that impact cell migration.

Main Methods:

  • High-content screening and data-driven analysis to identify EMT regulators.
  • Quantitative analysis of gene expression changes in response to miR614.
  • Functional testing of candidate genes (Miro1, TAPT1) involved in miR614-mediated migration.
  • Correlation analysis of TAPT1 expression with breast cancer patient relapse data.

Main Results:

  • Increased miR614 expression enhances both single-cell and collective migration.
  • miR614 specifically upregulates the EMT transcription factor Slug without inducing other canonical EMT factors.
  • Miro1 and TAPT1 were identified as miR614-suppressed genes that inhibit migration.
  • Suppression of Miro1 or TAPT1 increased Slug expression and cell migration rates.
  • Reduced TAPT1 expression correlated with an increased risk of relapse in breast cancer patients.

Conclusions:

  • miR614 is a novel regulator that enhances cell migration by increasing Slug expression.
  • Miro1 and TAPT1 are identified as key suppressors of migration regulated by miR614.
  • TAPT1 suppression is linked to increased breast cancer relapse, highlighting its clinical relevance.
  • These findings elucidate a mechanism involving miR614, TAPT1, and Miro1 in modulating EMT and breast cancer cell migration.