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Advances in Entomopathogen Isolation: A Case of Bacteria and Fungi

Lav Sharma1, Nitin Bohra2, Vishnu D Rajput3

  • 1Centre for the Research and Technology of Agro-Environment and Biological Sciences, Universidade de Trás-os-Montes e Alto Douro, Quinta de Prados, 5000-801 Vila Real, Portugal.

Microorganisms
|December 30, 2020
PubMed

Insights

This review summarizes methods for isolating entomopathogenic bacteria and fungi, crucial for developing microbial biopesticides. It covers techniques for important genera like Serratia, Bacillus, Beauveria, and Metarhizium.

Area of Science:

  • Microbiology
  • Entomology
  • Biopesticide Development

Background:

  • Entomopathogenic bacteria and fungi are prevalent in soil and insect cadavers.
  • Effective isolation techniques are essential for their application as biopesticides.
  • Previous research has explored various culture media and insect baiting methods.

Purpose of the Study:

  • To review isolation techniques for entomopathogenic microbes developed over the last 50 years.
  • To provide practical protocols for isolating key entomopathogenic bacteria and fungi.
  • To focus on economically important groups for the biopesticide market.

Main Methods:

  • Literature review of isolation techniques for entomopathogenic bacteria and fungi.
  • Compilation of protocols for specific microbial groups.
  • Selection of bacteria (Serratia spp., Bacilli) and fungi (Hypocreales order: Beauveria, Clonostachys, Lecanicillium, Metarhizium, Purpureocillium).

Main Results:

  • A comprehensive overview of established isolation methods is presented.
  • Protocols are detailed for isolating significant entomopathogenic bacteria and fungi.
  • The review highlights microbes with market relevance for biopesticides.

Conclusions:

  • Established and novel techniques facilitate the isolation of entomopathogenic microbes.
  • Standardized protocols are vital for consistent biopesticide development.
  • Focusing on market-relevant species accelerates the adoption of microbial control agents.
Keywords:
Bacillus thuringiensisBeauveriaHypocrealesMetarhiziumSerratia

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